CYP21A2 Antibody
Catalog Number: LS-C109472-100
100 µL (1 mg/mL) / $459.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CYP21A2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G87235-20
Catalog Number: LS-G87235
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CYP21A2 (NM_001128590) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 21-OHase | CAH1 | CYP21B | Cytochrome P450-C21 | CYP21A2 | Cytochrome P450 21 | Cytochrome P450 XXI | p450c21B | CA21H | CYP21 | Cytochrome P-450c21 | Cytochrome P450-C21B | Steroid 21-hydroxylase | Steroid 21-monooxygenase
- Origin Species
- Human
- NCBI GenBank
- NM_000500
- SwissProt
- P08686
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLLGLLLLL PLLAGARLLW NWWKLRSLHL PPLAPGFLHL LQPDLPIYLL GLTQKFGPIY61RLHLGLQDKL VSRNYPDLSL GDYSLLWKAH KKLTRSALLL GIRDSMEPVV EQLTQEFCER121MRAQPGTPVA IEEEFSLLTC SIICYLTFGD KIKDDNLMPA YYKCIQEVLK TWSHWSIQIV181DVIPFLRFFP NPGLRRLKQA IEKRDHIVEM QLRQHKESLV AGQWRDMMDY MLQGVAQPSM241EEGSGQLLEG HVHMAAVDLL IGGTETTANT LSWAVVFLLH HPEIQQRLQE ELDHELGPGA301SSSRVPYKDR ARLPLLNATI AEVLRLRPVV PLALPHRTTR PSSISGYDIP EGTVIIPNLQ361GAHLDETVWE RPHEFWPDRF LEPGKNSRAL AFGCGARVCL GEPLARLELF VVLTRLLQAF421TLLPSGDALP SLQPLPHCSV ILKMQPFQVR LQPRGMGAHS PGQSQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
465 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
