CSNK1A1 / CK1 Alpha Antibody
Catalog Number: LS-C317618
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CSNK1A1 / CK1 Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G75016-20
Catalog Number: LS-G75016
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Casein Kinase 1 alpha(CSNK1A1) (NM_001025105) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK1 alpha | CK1a | CK1alpha | Clock regulator kinase | CSNK1A1 | CK1 | Casein kinase 1 alpha | CKIa | CKIalpha | Down-regulated in lung cancer | HLCDGP1 | PRO2975 | Casein kinase 1 | alpha 1 | Casein kinase I alpha | Casein kinase I isoform alpha | CKI-alpha
- Origin Species
- Human
- NCBI GenBank
- NM_001892
- SwissProt
- P48729
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ KARHPQLLYE61SKLYKILQGG VGIPHIRWYG QEKDYNVLVM DLLGPSLEDL FNFCSRRFTM KTVLMLADQM121ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC NKCLESPVGK RKRSMTVSTS QDPSFSGLNQ181LFLIDFGLAK KYRDNRTRQH IPYREDKNLT GTARYASINA HLGIEQSRRD DMESLGYVLM241YFNRTSLPWQ GLKAATKKQK YEKISEKKMS TPVEVLCKGF PAEFAMYLNY CRGLRFEEAP301DYMYLRQLFR ILFRTLNHQY DYTFDWTMLK QKAAQQAASS SGQGQQAQTP TGKQTDKTKS361NMKGFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
365 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
