Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CSNK1A1 / CK1 Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121131-100
Catalog Number: LS-G121131
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Casein Kinase 1 alpha(CSNK1A1) (NM_001025105), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK1 alpha | CK1a | CK1alpha | Clock regulator kinase | CSNK1A1 | CK1 | Casein kinase 1 alpha | CKIa | CKIalpha | Down-regulated in lung cancer | HLCDGP1 | PRO2975 | Casein kinase 1 | alpha 1 | Casein kinase I alpha | Casein kinase I isoform alpha | CKI-alpha
- Origin Species
- Human
- NCBI GenBank
- NM_001892
- SwissProt
- P48729
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ KARHPQLLYE61SKLYKILQGG VGIPHIRWYG QEKDYNVLVM DLLGPSLEDL FNFCSRRFTM KTVLMLADQM121ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC NKCLESPVGK RKRSMTVSTS QDPSFSGLNQ181LFLIDFGLAK KYRDNRTRQH IPYREDKNLT GTARYASINA HLGIEQSRRD DMESLGYVLM241YFNRTSLPWQ GLKAATKKQK YEKISEKKMS TPVEVLCKGF PAEFAMYLNY CRGLRFEEAP301DYMYLRQLFR ILFRTLNHQY DYTFDWTMLK QKAAQQAASS SGQGQQAQTP TGKQTDKTKS361NMKGFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
365 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.