LSBio
Human CRF2-12 / IL28RA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CRF2-12 / IL28RA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all IFNLR1 antibodies →
$220.00each
Catalog Number: LS-G84597-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human IL28 Receptor alpha(IFNLR1) (NM_173064) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IFNLR | IL-28R1 | Interferon | lambda receptor 1 | IFNLR1 | IL28RA | CRF2-12 | CRF2/12 | LICR2
Origin Species
Human
NCBI GenBank
NM_170743
SwissProt
Q8IU57

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MAGPERWGPL LLCLLQAAPG RPRLAPPQNV TLLSQNFSVY LTWLPGLGNP QDVTYFVAYQ
61SSPTRRRWRE VEECAGTKEL LCSMMCLKKQ DLYNKFKGRV RTVSPSSKSP WVESEYLDYL
121FEVEPAPPVL VLTQTEEILS ANATYQLPPC MPPLDLKYEV AFWKEGAGNK TLFPVTPHGQ
181PVQITLQPAA SEHHCLSART IYTFSVPKYS KFSKPTCFLL EVPEANWAFL VLPSLLILLL
241VIAAGGVIWK TLMGNPWFQR AKMPRALELT RGVRPTPRVR APATQQTRWK KDLAEDEEEE
301DEEDTEDGVS FQPYIEPPSF LGQEHQAPGH SEAGGVDSGR PRAPLVPSEG SSAWDSSDRS
361WASTVDSSWD RAGSSGYLAE KGPGQGPGGD GHQESLPPPE FSKDSGFLEE LPEDNLSSWA
421TWGTLPPEPN LVPGGPPVSL QTLTFCWESS PEEEEEARES EIEDSDAGSW GAESTQRTED
481RGRTLGHYMA R
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

491 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.