Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CRF2-12 / IL28RA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105400-100
Catalog Number: LS-G105400
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IL28 Receptor alpha(IFNLR1) (NM_173064), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IFNLR | IL-28R1 | Interferon | lambda receptor 1 | IFNLR1 | IL28RA | CRF2-12 | CRF2/12 | LICR2
- Origin Species
- Human
- NCBI GenBank
- NM_170743
- SwissProt
- Q8IU57
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGPERWGPL LLCLLQAAPG RPRLAPPQNV TLLSQNFSVY LTWLPGLGNP QDVTYFVAYQ61SSPTRRRWRE VEECAGTKEL LCSMMCLKKQ DLYNKFKGRV RTVSPSSKSP WVESEYLDYL121FEVEPAPPVL VLTQTEEILS ANATYQLPPC MPPLDLKYEV AFWKEGAGNK TLFPVTPHGQ181PVQITLQPAA SEHHCLSART IYTFSVPKYS KFSKPTCFLL EVPEANWAFL VLPSLLILLL241VIAAGGVIWK TLMGNPWFQR AKMPRALELT RGVRPTPRVR APATQQTRWK KDLAEDEEEE301DEEDTEDGVS FQPYIEPPSF LGQEHQAPGH SEAGGVDSGR PRAPLVPSEG SSAWDSSDRS361WASTVDSSWD RAGSSGYLAE KGPGQGPGGD GHQESLPPPE FSKDSGFLEE LPEDNLSSWA421TWGTLPPEPN LVPGGPPVSL QTLTFCWESS PEEEEEARES EIEDSDAGSW GAESTQRTED481RGRTLGHYMA RSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
491 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.