LSBio
Human CPAMD8 / KIAA1283 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CPAMD8 / KIAA1283 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CPAMD8 antibodies →
$220.00each
Catalog Number: LS-G84983-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CPAMD8 (NM_015692) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CPAMD8 | KIAA1283 | K-CAP
Origin Species
Human
NCBI GenBank
AB033109
SwissProt
Q8IZJ3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
211.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPGALRGGC EQRAPGGGAR TAALGPWPWP SPGPAPSPGA APPRSRKMSG ALLWPLLPLL
61LLLLSARDGV RAAQPQAPGY LIAAPSVFRA GVEEVISVTI FNSPREVTVQ AQLVAQGEPV
121VQSQGAILDK GTIKLKVPTG LRGQALLKVW GRGWQAEEGP LFHNQTSVTV DGRGASVFIQ
181TDKPVYRPQH RVLISIFTVS PNLRPVNEKL EAYILDPRGS RMIEWRHLKP FCCGITNMSF
241PLSDQPVLGE WFIFVEMQGH AYNKSFEVQK YVLPKFELLI DPPRYIQDLD ACETGTVRAR
301YTFGKPVAGA LMINMTVNGV GYYSHEVGRP VLRTTKILGS RDFDICVRDM IPADVPEHFR
361GRVSIWAMVT SVDGSQQVAF DDSTPVQRQL VDIRYSKDTR KQFKPGLAYV GKVELSYPDG
421SPAEGVTVQI KAELTPKDNI YTSEVVSQRG LVGFEIPSIP TSAQHVWLET KVMALNGKPV
481GAQYLPSYLS LGSWYSPSQC YLQLQPPSHP LQVGEEAYFS VKSTCPCNFT LYYEVAARGN
541IVLSGQQPAH TTQQRSKRAA PALEKPIRLT HLSETEPPPA PEAEVDVCVT SLHLAVTPSM
601VPLGRLLVFY VRENGEGVAD SLQFAVETFF ENQVSVTYSA NETQPGEVVD LRIRAARGSC
661VCVAAVDKSV YLLRSGFRLT PAQVFQELED YDVSDSFGVS REDGPFWWAG LTAQRRRRSS
721VFPWPWGITK DSGFAFTETG LVVMTDRVSL NHRQDGGLYT DEAVPAFQPH TGSLVAVAPS
781RHPPRTEKRK RTFFPETWIW HCLNISDPSG EGTLSVKVPD SITSWVGEAV ALSTSQGLGI
841AEPSLLKTFK PFFVDFMLPA LIIRGEQVKI PLSVYNYMGT CAEVYMKLSV PKGIQFVGHP
901GKRHVTKKMC VAPGEAEPIW VVLSFSDLGL NNITAKALAY GDTNCCRDGR SSKHPEENHA
961DRRVPIGVDH VRRSVMVEAE GVPRAYTYSA FFCPSERVHI STPNKYEFQY VQRPLRLTRF
1021DVAVRAHNDA RVALSSGPQD TAGMIEIVLG GHQNTRSWIS TSKMGEPVAS AHTAKILSWD
1081EFRTFWISWR GGLIQVGHGP EPSNESVIVA WTLPRPPEVQ FIGFSTGWGS MGEFRIWRKM
1141EVDESYSEAF TLGVPHGAIP GSERATASII GDVMGPTLNH LNNLLRLPFG CGEQNMIHFA
1201PNVFVLKYLQ KTQQLSPEVE RETTDYLVQG YQRQLTYKRQ DGSYSAFGER DASGSMWLTA
1261FVLKSFAQAR SFIFVDPREL AAAKSWIIQQ QQADGSFLAV GRVLNKDIQG GIHGTVPLTA
1321YVVVALLETG TASEEERGST DKARHFLESA APLAMDPYSC ALTTYALTLL RSPAAPEALR
1381KLRSLAIMRD GVTHWSLSNS WDVDKGTFLS FSDRVSQSVV SAEVEMTAYA LLTYTLLGDV
1441AAALPVVKWL SQQRNALGGF SSTQDTCVAL QALAEYAILS YAGGINLTVS LASTNLDYQE
1501TFELHRTNQK VLQTAAIPSL PTGLFVSAKG DGCCLMQIDV TYNVPDPVAK PAFQLLVSLQ
1561EPEAQGRPPP MPASAAEGSR GDWPPADDDD PAADQHHQEY KVMLEVCTRW LHAGSSNMAV
1621LEVPLLSGFR ADIESLEQLL LDKHMGMKRY EVAGRRVLFY FDEIPSRCLT CVRFRALREC
1681VVGRTSALPV SVYDYYEPAF EATRFYNVST HSPLARELCA GPACNEVERA PARGPGWFPG
1741ESGPAVAPEE GAAIARCGCD HDCGAQGNPV CGSDGVVYAS ACRLREAACR QAAPLEPAPP
1801SCCALEQRLP ASSSSTYGDD LASVAPGPLQ QDVKLNGAGL EVEDSDPEPE GEAEDRVTAG
1861PRPPVSSGNL ESSTQSASPF HRWGQTPAPQ RHSGRVVGAH RPGLLSPVFV YSPAFQSGGE
1921EGLWMSNTCT LR
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1932 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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