Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CPAMD8 / KIAA1283 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113057-100
Catalog Number: LS-G113057
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPAMD8 (NM_015692), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CPAMD8 | KIAA1283 | K-CAP
- Origin Species
- Human
- NCBI GenBank
- AB033109
- SwissProt
- Q8IZJ3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 211.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPGALRGGC EQRAPGGGAR TAALGPWPWP SPGPAPSPGA APPRSRKMSG ALLWPLLPLL61LLLLSARDGV RAAQPQAPGY LIAAPSVFRA GVEEVISVTI FNSPREVTVQ AQLVAQGEPV121VQSQGAILDK GTIKLKVPTG LRGQALLKVW GRGWQAEEGP LFHNQTSVTV DGRGASVFIQ181TDKPVYRPQH RVLISIFTVS PNLRPVNEKL EAYILDPRGS RMIEWRHLKP FCCGITNMSF241PLSDQPVLGE WFIFVEMQGH AYNKSFEVQK YVLPKFELLI DPPRYIQDLD ACETGTVRAR301YTFGKPVAGA LMINMTVNGV GYYSHEVGRP VLRTTKILGS RDFDICVRDM IPADVPEHFR361GRVSIWAMVT SVDGSQQVAF DDSTPVQRQL VDIRYSKDTR KQFKPGLAYV GKVELSYPDG421SPAEGVTVQI KAELTPKDNI YTSEVVSQRG LVGFEIPSIP TSAQHVWLET KVMALNGKPV481GAQYLPSYLS LGSWYSPSQC YLQLQPPSHP LQVGEEAYFS VKSTCPCNFT LYYEVAARGN541IVLSGQQPAH TTQQRSKRAA PALEKPIRLT HLSETEPPPA PEAEVDVCVT SLHLAVTPSM601VPLGRLLVFY VRENGEGVAD SLQFAVETFF ENQVSVTYSA NETQPGEVVD LRIRAARGSC661VCVAAVDKSV YLLRSGFRLT PAQVFQELED YDVSDSFGVS REDGPFWWAG LTAQRRRRSS721VFPWPWGITK DSGFAFTETG LVVMTDRVSL NHRQDGGLYT DEAVPAFQPH TGSLVAVAPS781RHPPRTEKRK RTFFPETWIW HCLNISDPSG EGTLSVKVPD SITSWVGEAV ALSTSQGLGI841AEPSLLKTFK PFFVDFMLPA LIIRGEQVKI PLSVYNYMGT CAEVYMKLSV PKGIQFVGHP901GKRHVTKKMC VAPGEAEPIW VVLSFSDLGL NNITAKALAY GDTNCCRDGR SSKHPEENHA961DRRVPIGVDH VRRSVMVEAE GVPRAYTYSA FFCPSERVHI STPNKYEFQY VQRPLRLTRF1021DVAVRAHNDA RVALSSGPQD TAGMIEIVLG GHQNTRSWIS TSKMGEPVAS AHTAKILSWD1081EFRTFWISWR GGLIQVGHGP EPSNESVIVA WTLPRPPEVQ FIGFSTGWGS MGEFRIWRKM1141EVDESYSEAF TLGVPHGAIP GSERATASII GDVMGPTLNH LNNLLRLPFG CGEQNMIHFA1201PNVFVLKYLQ KTQQLSPEVE RETTDYLVQG YQRQLTYKRQ DGSYSAFGER DASGSMWLTA1261FVLKSFAQAR SFIFVDPREL AAAKSWIIQQ QQADGSFLAV GRVLNKDIQG GIHGTVPLTA1321YVVVALLETG TASEEERGST DKARHFLESA APLAMDPYSC ALTTYALTLL RSPAAPEALR1381KLRSLAIMRD GVTHWSLSNS WDVDKGTFLS FSDRVSQSVV SAEVEMTAYA LLTYTLLGDV1441AAALPVVKWL SQQRNALGGF SSTQDTCVAL QALAEYAILS YAGGINLTVS LASTNLDYQE1501TFELHRTNQK VLQTAAIPSL PTGLFVSAKG DGCCLMQIDV TYNVPDPVAK PAFQLLVSLQ1561EPEAQGRPPP MPASAAEGSR GDWPPADDDD PAADQHHQEY KVMLEVCTRW LHAGSSNMAV1621LEVPLLSGFR ADIESLEQLL LDKHMGMKRY EVAGRRVLFY FDEIPSRCLT CVRFRALREC1681VVGRTSALPV SVYDYYEPAF EATRFYNVST HSPLARELCA GPACNEVERA PARGPGWFPG1741ESGPAVAPEE GAAIARCGCD HDCGAQGNPV CGSDGVVYAS ACRLREAACR QAAPLEPAPP1801SCCALEQRLP ASSSSTYGDD LASVAPGPLQ QDVKLNGAGL EVEDSDPEPE GEAEDRVTAG1861PRPPVSSGNL ESSTQSASPF HRWGQTPAPQ RHSGRVVGAH RPGLLSPVFV YSPAFQSGGE1921EGLWMSNTCT LRSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1932 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.