LSBio
Human COL9A1 / Collagen IX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human COL9A1 / Collagen IX Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all COL9A1 antibodies →
$153.00each
Catalog Number: LS-G75243-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Collagen IX(COL9A1) (NM_078485) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Alpha-1(IX) collagen chain | COL9A1 | Collagen alpha-1(IX) chain | Collagen | type IX | alpha 1 | EDM6 | MED | STL4 | DJ149L1.1.2
Origin Species
Human
NCBI GenBank
NM_001851

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAWTARDRGA LGLLLLGLCL CAAQRGPPGE QGPPGPPGPP GVPGIDGIDG DRGPKGPPGP
61PGPAGEPGKP GAPGKPGTPG ADGLTGPDGS PGSIGSKGQK GEPGVPGSRG FPGRGIPGPP
121GPPGTAGLPG ELGRVGPVGD PGRRGPPGPP GPPGPRGTIG FHDGDPLCPN ACPPGRSGYP
181GLPGMRGHKG AKGEIGEPGR QGHKGEEGDQ GELGEVGAQG PPGAQGLRGI TGIVGDKGEK
241GARGLDGEPG PQGLPGAPGD QGQRGPPGEA GPKGDRGAEG ARGIPGLPGP KGDTGLPGVD
301GRDGIPGMPG TKGEPGKPGP PGDAGLQGLP GVPGIPGAKG VAGEKGSTGA PGKPGQMGNS
361GKPGQQGPPG EVGPRGPRGL PGSRGELGPV GSPGLPGKLG SLGSPGLPGL PGPPGLPGMK
421GDRGVVGEPG PKGEQGASGE EGEAGERGEL GDIGLPGPKG SAGNPGEPGL RGPEGSRGLP
481GVEGPRGPPG PRGVQGEQGA TGLPGVQGPP GRAPTDQHIK QVCMRVIQEH FAEMAASLKR
541PDSGATGLPG RPGPPGPPGP PGENGFPGQM GIRGLPGIKG PPGALGLRGP KGDLGEKGER
601GPPGRGPNGL PGAIGLPGDP GPASYGRNGR DGERGPPGVA GIPGVPGPPG PPGLPGFCEP
661ASCTMQAGQR AFNKGPDP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

678 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.