Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COL9A1 / Collagen IX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107896-100
Catalog Number: LS-G107896
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Collagen IX(COL9A1) (NM_078485), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha-1(IX) collagen chain | COL9A1 | Collagen alpha-1(IX) chain | Collagen | type IX | alpha 1 | EDM6 | MED | STL4 | DJ149L1.1.2
- Origin Species
- Human
- NCBI GenBank
- NM_001851
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAWTARDRGA LGLLLLGLCL CAAQRGPPGE QGPPGPPGPP GVPGIDGIDG DRGPKGPPGP61PGPAGEPGKP GAPGKPGTPG ADGLTGPDGS PGSIGSKGQK GEPGVPGSRG FPGRGIPGPP121GPPGTAGLPG ELGRVGPVGD PGRRGPPGPP GPPGPRGTIG FHDGDPLCPN ACPPGRSGYP181GLPGMRGHKG AKGEIGEPGR QGHKGEEGDQ GELGEVGAQG PPGAQGLRGI TGIVGDKGEK241GARGLDGEPG PQGLPGAPGD QGQRGPPGEA GPKGDRGAEG ARGIPGLPGP KGDTGLPGVD301GRDGIPGMPG TKGEPGKPGP PGDAGLQGLP GVPGIPGAKG VAGEKGSTGA PGKPGQMGNS361GKPGQQGPPG EVGPRGPRGL PGSRGELGPV GSPGLPGKLG SLGSPGLPGL PGPPGLPGMK421GDRGVVGEPG PKGEQGASGE EGEAGERGEL GDIGLPGPKG SAGNPGEPGL RGPEGSRGLP481GVEGPRGPPG PRGVQGEQGA TGLPGVQGPP GRAPTDQHIK QVCMRVIQEH FAEMAASLKR541PDSGATGLPG RPGPPGPPGP PGENGFPGQM GIRGLPGIKG PPGALGLRGP KGDLGEKGER601GPPGRGPNGL PGAIGLPGDP GPASYGRNGR DGERGPPGVA GIPGVPGPPG PPGLPGFCEP661ASCTMQAGQR AFNKGPDPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
678 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.