COL28A1 Antibody
Catalog Number: LS-C743344
2 sizes / from $341.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human COL28A1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95955-20
Catalog Number: LS-G95955
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Collagen alpha 1(XXVIII) chain(COL28A1) (NM_001037763) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001037763
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 116.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWNRYFVFYL LLLSAFTSQT VSGQRKKGPK SNLLARKSDV QGSICFIDIV FIVDSSESSK61IALFDKQKDF VDSLSDKIFQ LTPGRSLEYD IKLAALQFSS SVQIDPPFSS WKDLQTFKQK121VKSMNLIGQG TFSYYAISNA TRLLKREGRK DGVKVVLLMT DGIDHPKNPD VQSISEDARI181SGISFITIAL STVVNEAKLR LISGDSSSEP TLLLSDPTLV DKIQDRLDIL FEKKCERKIC241ECEKGDPGDP GPPGTHGNPG IKGERGPKGN PGNAQKGEAG ERGPGGIPGY KGDKGERGEC301GKPGIKGDKG SPGPYGPKGP RGIQGITGPP GDPGPKGFQG NKGEPGPPGP YGSPGAPGIG361QQGIKGERGQ EGRPGAPGPI GVGEPGQPGP RGPEGVPGER GLPGEGFPGP KGEKGSEGPT421GPQGLQGLSI KGEKGDIGPV GPQGPMGIPG IGSQGEQGIQ GPIGPPGPQG PAGQGLPGSK481GEVGQMGPTG PRGPVGIGVQ GPKGEPGSIG LPGQPGVPGE DGAAGKKGEA GLPGARGPEG541PPGKGQPGPK GDEGKKGSKG NQGQRGLPGP EGPKGEPGIM GPFGMPGTSI PGPPGPKGDR601GGPGIPGFKG EPGLSIRGPK GVQGPRGPVG APGLKGDGYP GVPGPRGLPG PPGPMGLRGV661GDTGAKGEPG VRGPPGPSGP RGVGTQGPKG DTGQKGLPGP PGPPGYGSQG IKGEQGPQGF721PGPKGTMGHG LPGQKGEHGE RGDVGKKGDK GEIGEPGSPG KQGLQGPKGD LGLTKEEIIK781LITEICGCGP KCKETPLELV FVIDSSESVG PENFQIIKNF VKTMADRVAL DLATARIGII841NYSHKVEKVA NLKQFSSKDD FKLAVDNMQY LGEGTYTATA LQAANDMFED ARPGVKKVAL901VITDGQTDSR DKEKLTEVVK NASDTNVEIF VIGVVKKNDP NFEIFHKEMN LIATDPEHVY961QFDDFFTLQD TLKQKLFQKI CEDFDSYLVQ IFGSSSPQPG FGMSGEELSE STPEPQKEIS1021ESLSVTRDQD EDDKAPEPTW ADDLPATTSS EATTTPRPLL STPVDGAEDP RCLEALKPGN1081CGEYVVRWYY DKQVNSCARF WFSGCNGSGN RFNSEKECQE TCIQGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1125 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
