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COL28A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

COL28A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all COL28A1 antibodies →
$725.00each
Catalog Number: LS-G120540-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Collagen alpha 1(XXVIII) chain(COL28A1) (NM_001037763), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001037763

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWNRYFVFYL LLLSAFTSQT VSGQRKKGPK SNLLARKSDV QGSICFIDIV FIVDSSESSK
61IALFDKQKDF VDSLSDKIFQ LTPGRSLEYD IKLAALQFSS SVQIDPPFSS WKDLQTFKQK
121VKSMNLIGQG TFSYYAISNA TRLLKREGRK DGVKVVLLMT DGIDHPKNPD VQSISEDARI
181SGISFITIAL STVVNEAKLR LISGDSSSEP TLLLSDPTLV DKIQDRLDIL FEKKCERKIC
241ECEKGDPGDP GPPGTHGNPG IKGERGPKGN PGNAQKGEAG ERGPGGIPGY KGDKGERGEC
301GKPGIKGDKG SPGPYGPKGP RGIQGITGPP GDPGPKGFQG NKGEPGPPGP YGSPGAPGIG
361QQGIKGERGQ EGRPGAPGPI GVGEPGQPGP RGPEGVPGER GLPGEGFPGP KGEKGSEGPT
421GPQGLQGLSI KGEKGDIGPV GPQGPMGIPG IGSQGEQGIQ GPIGPPGPQG PAGQGLPGSK
481GEVGQMGPTG PRGPVGIGVQ GPKGEPGSIG LPGQPGVPGE DGAAGKKGEA GLPGARGPEG
541PPGKGQPGPK GDEGKKGSKG NQGQRGLPGP EGPKGEPGIM GPFGMPGTSI PGPPGPKGDR
601GGPGIPGFKG EPGLSIRGPK GVQGPRGPVG APGLKGDGYP GVPGPRGLPG PPGPMGLRGV
661GDTGAKGEPG VRGPPGPSGP RGVGTQGPKG DTGQKGLPGP PGPPGYGSQG IKGEQGPQGF
721PGPKGTMGHG LPGQKGEHGE RGDVGKKGDK GEIGEPGSPG KQGLQGPKGD LGLTKEEIIK
781LITEICGCGP KCKETPLELV FVIDSSESVG PENFQIIKNF VKTMADRVAL DLATARIGII
841NYSHKVEKVA NLKQFSSKDD FKLAVDNMQY LGEGTYTATA LQAANDMFED ARPGVKKVAL
901VITDGQTDSR DKEKLTEVVK NASDTNVEIF VIGVVKKNDP NFEIFHKEMN LIATDPEHVY
961QFDDFFTLQD TLKQKLFQKI CEDFDSYLVQ IFGSSSPQPG FGMSGEELSE STPEPQKEIS
1021ESLSVTRDQD EDDKAPEPTW ADDLPATTSS EATTTPRPLL STPVDGAEDP RCLEALKPGN
1081CGEYVVRWYY DKQVNSCARF WFSGCNGSGN RFNSEKECQE TCIQG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1125 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.