LSBio
Human COL20A1 / Collagen XX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human COL20A1 / Collagen XX Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all COL20A1 antibodies →
$153.00each
Catalog Number: LS-G86890-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human COL20A1 (NM_020882) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA261N11.4 | Collagen | type XX | alpha 1 | Collagen-like protein | COL20A1 | Collagen alpha-1(XX) chain | KIAA1510
Origin Species
Human
NCBI GenBank
AB040943
SwissProt
Q9P218

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
135.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSGDPAHLG LCLWLWLGAT LGREQVQASG LLRLAVLPED RLQMKWRESE GSGLGYLVQV
61KPMAGDSEQE VILTTKTPKA TVGGLSPSKG YTLQIFELTG SGRFLLARRE FVIEDLKSSS
121LDRSSQRPLG SGAPEPTPSH TGSPDPEQAS EPQVAFTPSQ DPRTPAGPQF RCLPPVPADM
181VFLVDGSWSI GHSHFQQVKD FLASVIAPFE IGPDKVQVGL TQYSGDAQTE WDLNSLSTKE
241QVLAAVRRLR YKGGNTFTGL ALTHVLGQNL QPAAGLRPEA AKVVILVTDG KSQDDVHTAA
301RVLKDLGVNV FAVGVKNADE AELRLLASPP RDITVHSVLD FLQLGALAGL LSRLICQRLQ
361GGSPRQGPAA APALDTLPAP TSLVLSQVTS SSIRLSWTPA PRHPLKYLIV WRASRGGTPR
421EVVVEGPAAS TELHNLASRT EYLVSVFPIY EGGVGEGLRG LVTTAPLPPP RALTLAAVTP
481RTVHLTWQPS AGATHYLVRC SPASPKGEEE EREVQVGRPE VLLDGLEPGR DYEVSVQSLR
541GPEGSEARGI RARTPTLAPP RHLGFSDVSH DAARVFWEGA PRPVRLVRVT YVSSEGGHSG
601QTEAPGNATS ATLGPLSSST TYTVRVTCLY PGGGSSTLTG RVTTKKAPSP SQLSMTELPG
661DAVQLAWVAA APSGVLVYQI TWTPLGEGKA HEISVPGNLG TAVLPGLGRH TEYDVTILAY
721YRDGARSDPV SLRYTPSTVS RSPPSNLALA SETPDSLQVS WTPPLGRVLH YWLTYAPASG
781LGPEKSVSVP GARSHVTLPD LQAATKYRVL VSAIYAAGRS EAVSATGQTA CPALRPDGSL
841PGFDLMVAFS LVEKAYASIR GVAMEPSAFG GTPTFTLFKD AQLTRRVSDV YPAPLPPEHT
901IVFLVRLLPE TPREAFALWQ MTAEDFQPLL GVLLDAGKKS LTYFHRDPRA ALQEATFDPQ
961EVRKIFFGSF HKVHVAVGRS KVRLYVDCRK VAERPLGEMG SPPAAGFVTL GRLAKARGPR
1021SSSAAFQLQM LQIVCSDTWA DEDRCCELPA SRDGETCPAF VSACSCSSET PGPPGPQGPP
1081GLPGRNGTPG EQGFPGPRGP PGVKGEKGDH GLPGLQGHPG HQGIPGRVGL QGPKGMRGLE
1141GTAGLPGPPG PRGFQGMAGA RGTSGERGPP GTVGPTGLPG PKGERGEKGE PQSLATLYQL
1201VSQASHVSKF DSFHENTRPP MPILEQKLEP GTEPLGSPGT RSKALVPGEW GRGGRHLEGR
1261GEPGAVGQMG SPGQQGASTQ GLWE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1284 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.