Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COL20A1 / Collagen XX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110928-100
Catalog Number: LS-G110928
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COL20A1 (NM_020882), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA261N11.4 | Collagen | type XX | alpha 1 | Collagen-like protein | COL20A1 | Collagen alpha-1(XX) chain | KIAA1510
- Origin Species
- Human
- NCBI GenBank
- AB040943
- SwissProt
- Q9P218
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 135.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSGDPAHLG LCLWLWLGAT LGREQVQASG LLRLAVLPED RLQMKWRESE GSGLGYLVQV61KPMAGDSEQE VILTTKTPKA TVGGLSPSKG YTLQIFELTG SGRFLLARRE FVIEDLKSSS121LDRSSQRPLG SGAPEPTPSH TGSPDPEQAS EPQVAFTPSQ DPRTPAGPQF RCLPPVPADM181VFLVDGSWSI GHSHFQQVKD FLASVIAPFE IGPDKVQVGL TQYSGDAQTE WDLNSLSTKE241QVLAAVRRLR YKGGNTFTGL ALTHVLGQNL QPAAGLRPEA AKVVILVTDG KSQDDVHTAA301RVLKDLGVNV FAVGVKNADE AELRLLASPP RDITVHSVLD FLQLGALAGL LSRLICQRLQ361GGSPRQGPAA APALDTLPAP TSLVLSQVTS SSIRLSWTPA PRHPLKYLIV WRASRGGTPR421EVVVEGPAAS TELHNLASRT EYLVSVFPIY EGGVGEGLRG LVTTAPLPPP RALTLAAVTP481RTVHLTWQPS AGATHYLVRC SPASPKGEEE EREVQVGRPE VLLDGLEPGR DYEVSVQSLR541GPEGSEARGI RARTPTLAPP RHLGFSDVSH DAARVFWEGA PRPVRLVRVT YVSSEGGHSG601QTEAPGNATS ATLGPLSSST TYTVRVTCLY PGGGSSTLTG RVTTKKAPSP SQLSMTELPG661DAVQLAWVAA APSGVLVYQI TWTPLGEGKA HEISVPGNLG TAVLPGLGRH TEYDVTILAY721YRDGARSDPV SLRYTPSTVS RSPPSNLALA SETPDSLQVS WTPPLGRVLH YWLTYAPASG781LGPEKSVSVP GARSHVTLPD LQAATKYRVL VSAIYAAGRS EAVSATGQTA CPALRPDGSL841PGFDLMVAFS LVEKAYASIR GVAMEPSAFG GTPTFTLFKD AQLTRRVSDV YPAPLPPEHT901IVFLVRLLPE TPREAFALWQ MTAEDFQPLL GVLLDAGKKS LTYFHRDPRA ALQEATFDPQ961EVRKIFFGSF HKVHVAVGRS KVRLYVDCRK VAERPLGEMG SPPAAGFVTL GRLAKARGPR1021SSSAAFQLQM LQIVCSDTWA DEDRCCELPA SRDGETCPAF VSACSCSSET PGPPGPQGPP1081GLPGRNGTPG EQGFPGPRGP PGVKGEKGDH GLPGLQGHPG HQGIPGRVGL QGPKGMRGLE1141GTAGLPGPPG PRGFQGMAGA RGTSGERGPP GTVGPTGLPG PKGERGEKGE PQSLATLYQL1201VSQASHVSKF DSFHENTRPP MPILEQKLEP GTEPLGSPGT RSKALVPGEW GRGGRHLEGR1261GEPGAVGQMG SPGQQGASTQ GLWETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1284 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.