LSBio
Human COL19A1 / Collagen XIX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human COL19A1 / Collagen XIX Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all COL19A1 antibodies →
$220.00each
Catalog Number: LS-G80885-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human COL19A1 (NM_001858) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
A1 chain of type XIX collagen | COL9A1L | Collagen alpha 1 (Y) chain | COL19A1 | D6S228E | Collagen alpha-1(XIX) chain | Collagen alpha-1(Y) chain | Collagen | type XIX | alpha 1
Origin Species
Human
NCBI GenBank
NM_001858
SwissProt
Q14993

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115.22 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRLTGPWKLW LWMSIFLLPA STSVTVRDKT EESCPILRIE GHQLTYDNIN KLEVSGFDLG
61DSFSLRRAFC ESDKTCFKLG SALLIRDTIK IFPKGLPEEY SVAAMFRVRR NAKKERWFLW
121QVLNQQNIPQ ISIVVDGGKK VVEFMFQATE GDVLNYIFRN RELRPLFDRQ WHKLGISIQS
181QVISLYMDCN LIARRQTDEK DTVDFHGRTV IATRASDGKP VDIELHQLKI YCSANLIAQE
241TCCEISDTKC PEQDGFGNIA SSWVTAHASK MSSYLPAKQE LKDQCQCIPN KGEAGLPGAP
301GSPGQKGHKG EPGENGLHGA PGFPGQKGEQ GFEGSKGETG EKGEQGEKGD PALAGLNGEN
361GLKGDLGPHG PPGPKGEKGD TGPPGPPALP GSLGIQGPQG PPGKEGQRGR RGKTGPPGKP
421GPPGPPGPPG IQGIHQTLGG YYNKDNKGND EHEAGGLKGD KGETGLPGFP GSVGPKGQKG
481EPGEPFTKGE KGDRGEPGVI GSQGVKGEPG DPGPPGLIGS PGLKGQQGSA GSMGPRGPPG
541DVGLPGEHGI PGKQGIKGEK GDPGGIIGPP GLPGPKGEAG PPGKSLPGEP GLDGNPGAPG
601PRGPKGERGL PGVHGSPGDI GPQGIGIPGR TGAQGPAGEP GIQGPRGLPG LPGTPGTPGN
661DGVPGRDGKP GLPGPPGDPI ALPLLGDIGA LLKNFCGNCQ ASVPGLKSNK GEEGGAGEPG
721KYDSMARKGD IGPRGPPGIP GREGPKGSKG ERGYPGIPGE KGDEGLQGIP GIPGAPGPTG
781PPGLMGRTGH PGPTGAKGEK GSDGPPGKPG PPGPPGIPFN ERNGMSSLYK IKGGVNVPSY
841PGPPGPPGPK GDPGPVGEPG AMGLPGLEGF PGVKGDRGPA GPPGIAGMSG KPGAPGPPGV
901PGEPGERGPV GDIGFPGPEG PSGKPGINGK DGIPGAQGIM GKPGDRGPKG ERGDQGIPGD
961RGSQGERGKP GLTGMKGAIG PMGPPGNKGS MGSPGHQGPP GSPGIPGIPA DAVSFEEIKK
1021YINQEVLRIF EERMAVFLSQ LKLPAAMLAA QAYGRPGPPG KDGLPGPPGD PGPQGYRGQK
1081GERGEPGIGL PGSPGLPGTS ALGLPGSPGA PGPQGPPGPS GRCNPEDCLY PVSHAHQRTG
1141GN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1142 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.