Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COL19A1 / Collagen XIX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118367-100
Catalog Number: LS-G118367
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COL19A1 (NM_001858), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A1 chain of type XIX collagen | COL9A1L | Collagen alpha 1 (Y) chain | COL19A1 | D6S228E | Collagen alpha-1(XIX) chain | Collagen alpha-1(Y) chain | Collagen | type XIX | alpha 1
- Origin Species
- Human
- NCBI GenBank
- NM_001858
- SwissProt
- Q14993
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 115.22 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRLTGPWKLW LWMSIFLLPA STSVTVRDKT EESCPILRIE GHQLTYDNIN KLEVSGFDLG61DSFSLRRAFC ESDKTCFKLG SALLIRDTIK IFPKGLPEEY SVAAMFRVRR NAKKERWFLW121QVLNQQNIPQ ISIVVDGGKK VVEFMFQATE GDVLNYIFRN RELRPLFDRQ WHKLGISIQS181QVISLYMDCN LIARRQTDEK DTVDFHGRTV IATRASDGKP VDIELHQLKI YCSANLIAQE241TCCEISDTKC PEQDGFGNIA SSWVTAHASK MSSYLPAKQE LKDQCQCIPN KGEAGLPGAP301GSPGQKGHKG EPGENGLHGA PGFPGQKGEQ GFEGSKGETG EKGEQGEKGD PALAGLNGEN361GLKGDLGPHG PPGPKGEKGD TGPPGPPALP GSLGIQGPQG PPGKEGQRGR RGKTGPPGKP421GPPGPPGPPG IQGIHQTLGG YYNKDNKGND EHEAGGLKGD KGETGLPGFP GSVGPKGQKG481EPGEPFTKGE KGDRGEPGVI GSQGVKGEPG DPGPPGLIGS PGLKGQQGSA GSMGPRGPPG541DVGLPGEHGI PGKQGIKGEK GDPGGIIGPP GLPGPKGEAG PPGKSLPGEP GLDGNPGAPG601PRGPKGERGL PGVHGSPGDI GPQGIGIPGR TGAQGPAGEP GIQGPRGLPG LPGTPGTPGN661DGVPGRDGKP GLPGPPGDPI ALPLLGDIGA LLKNFCGNCQ ASVPGLKSNK GEEGGAGEPG721KYDSMARKGD IGPRGPPGIP GREGPKGSKG ERGYPGIPGE KGDEGLQGIP GIPGAPGPTG781PPGLMGRTGH PGPTGAKGEK GSDGPPGKPG PPGPPGIPFN ERNGMSSLYK IKGGVNVPSY841PGPPGPPGPK GDPGPVGEPG AMGLPGLEGF PGVKGDRGPA GPPGIAGMSG KPGAPGPPGV901PGEPGERGPV GDIGFPGPEG PSGKPGINGK DGIPGAQGIM GKPGDRGPKG ERGDQGIPGD961RGSQGERGKP GLTGMKGAIG PMGPPGNKGS MGSPGHQGPP GSPGIPGIPA DAVSFEEIKK1021YINQEVLRIF EERMAVFLSQ LKLPAAMLAA QAYGRPGPPG KDGLPGPPGD PGPQGYRGQK1081GERGEPGIGL PGSPGLPGTS ALGLPGSPGA PGPQGPPGPS GRCNPEDCLY PVSHAHQRTG1141GNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1142 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.