COBL Antibody (aa184-211)
Catalog Number: LS-C166907-400
400 µL / $401.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human COBL Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G83325-20
Catalog Number: LS-G83325
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Cordon bleu homolog(COBL) (NM_015198) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COBL | Cordon-bleu homolog (mouse) | Cordon-bleu WH2 repeat protein | KIAA0633 | Protein cordon-bleu
- Origin Species
- Human
- NCBI GenBank
- AA594937
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 135.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDAPRASAAK PPTGRKMKAR APPPPGKAAT LHVHSDQKPP HDGALGSQQN LVRMKEALRA61STMDVTVVLP SGLEKRSVLN GSHAMMDLLV ELCLQNHLNP SHHALEIRSS ETQQPLSFKP121NTLIGTLNVH TVFLKEKVPE EKVKPGPPKV PEKSVRLVVN YLRTQKAVVR VSPEVPLQNI181LPVICAKCEV SPEHVVLLRD NIAGEELELS KSLNELGIKE LYAWDNRRET FRKSSLGNDE241TDKEKKKFLG FFKVNKRSNS KGCLTTPNSP SMHSRSLTLG PSLSLGSISG VSVKSEMKKR301RAPPPPGSGP PVQDKASEKV SLGSQIDLQK KKRRAPAPPP PQPPPPSPLI PNRTEDKEEN361RKSTMVSLPL GSGSHCSPDG APQVLSEAEE TVSVGSCFAS EDTTEDSGVM SSPSDIVSLD421SQQDSMKYKD KWATDQEDCS DQDLAGTPDL GPQKSPLWEK NGSENSHLRT EKAVTASNDE481EDLLIAGEFR KTLAELDEDL EEMEDSYETD TSSLTSSIHG ASNHCPQDAM IPHGDTDAIP541VTFIGEVSDD PVDSGLFSNR NNNAGSFDSE GVASRRDSLA PLQAEHSQPH EKAREEVPAL601HPASHDVGKG IRVALSNISK DGNLMETAPR VTSFASNLHT DNLNAKVKDK VYGCADGERT661QATERVNSQP VNEKDSNDKN AALAPTSWHQ RGQNPGKSYR LKHGLTTYKI IPPKSEMRCY721DRDVSLSTGA IKIDELGNLV SPHATGIRII SLSSSVPEAE SQPIGKVREF WRCNSVEKHL781GRPSESSARG PPSTPVPTQT QNPESRLQAD PKPISPQQKS AHHEGRNPLG EGRNQPPTMG841MGHVRVPAAH TTEVTFLKPQ RRTSSQYVAS AIAKRIGAPK VHADVVRPHG YAEKGYAGKA901PVLAAPPVTV KDDRTSSPHS ETQGWKDGAQ WPCVTPPNNH GEDLAVGAPP RGEVIGPHRK961LSTQDRPAAI HRSSCFSLVQ SSQRDRVSVG QSCGFSGKQS TSSQEASSAS EPRRAPDGTD1021PPPPHTSDTQ ACSRELVNGS VRAPGHGEPS HPPGGSGTES HILLEREEKP SVFSTDGNET1081DSIWPPSIFG PKKKFKPVVQ RPVPKDTSLH SALMEAIHSA GGKDRLRKTA EHTGEGRPAK1141LSYTEAEGER SALLAAIRGH SGTCSLRKVA SSASEELQSF RDAALSAQGS ESPLLEDLGL1201LSPPAIPPPP PPPSQALSAP RTASRFSTGT LSNTADARQA LMDAIRSGTG AARLRKVPLL1261VSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1261 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
