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COBL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

COBL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all COBL antibodies →
$725.00each
Catalog Number: LS-G113389-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cordon bleu homolog(COBL) (NM_015198), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
COBL | Cordon-bleu homolog (mouse) | Cordon-bleu WH2 repeat protein | KIAA0633 | Protein cordon-bleu
Origin Species
Human
NCBI GenBank
AA594937

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
135.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDAPRASAAK PPTGRKMKAR APPPPGKAAT LHVHSDQKPP HDGALGSQQN LVRMKEALRA
61STMDVTVVLP SGLEKRSVLN GSHAMMDLLV ELCLQNHLNP SHHALEIRSS ETQQPLSFKP
121NTLIGTLNVH TVFLKEKVPE EKVKPGPPKV PEKSVRLVVN YLRTQKAVVR VSPEVPLQNI
181LPVICAKCEV SPEHVVLLRD NIAGEELELS KSLNELGIKE LYAWDNRRET FRKSSLGNDE
241TDKEKKKFLG FFKVNKRSNS KGCLTTPNSP SMHSRSLTLG PSLSLGSISG VSVKSEMKKR
301RAPPPPGSGP PVQDKASEKV SLGSQIDLQK KKRRAPAPPP PQPPPPSPLI PNRTEDKEEN
361RKSTMVSLPL GSGSHCSPDG APQVLSEAEE TVSVGSCFAS EDTTEDSGVM SSPSDIVSLD
421SQQDSMKYKD KWATDQEDCS DQDLAGTPDL GPQKSPLWEK NGSENSHLRT EKAVTASNDE
481EDLLIAGEFR KTLAELDEDL EEMEDSYETD TSSLTSSIHG ASNHCPQDAM IPHGDTDAIP
541VTFIGEVSDD PVDSGLFSNR NNNAGSFDSE GVASRRDSLA PLQAEHSQPH EKAREEVPAL
601HPASHDVGKG IRVALSNISK DGNLMETAPR VTSFASNLHT DNLNAKVKDK VYGCADGERT
661QATERVNSQP VNEKDSNDKN AALAPTSWHQ RGQNPGKSYR LKHGLTTYKI IPPKSEMRCY
721DRDVSLSTGA IKIDELGNLV SPHATGIRII SLSSSVPEAE SQPIGKVREF WRCNSVEKHL
781GRPSESSARG PPSTPVPTQT QNPESRLQAD PKPISPQQKS AHHEGRNPLG EGRNQPPTMG
841MGHVRVPAAH TTEVTFLKPQ RRTSSQYVAS AIAKRIGAPK VHADVVRPHG YAEKGYAGKA
901PVLAAPPVTV KDDRTSSPHS ETQGWKDGAQ WPCVTPPNNH GEDLAVGAPP RGEVIGPHRK
961LSTQDRPAAI HRSSCFSLVQ SSQRDRVSVG QSCGFSGKQS TSSQEASSAS EPRRAPDGTD
1021PPPPHTSDTQ ACSRELVNGS VRAPGHGEPS HPPGGSGTES HILLEREEKP SVFSTDGNET
1081DSIWPPSIFG PKKKFKPVVQ RPVPKDTSLH SALMEAIHSA GGKDRLRKTA EHTGEGRPAK
1141LSYTEAEGER SALLAAIRGH SGTCSLRKVA SSASEELQSF RDAALSAQGS ESPLLEDLGL
1201LSPPAIPPPP PPPSQALSAP RTASRFSTGT LSNTADARQA LMDAIRSGTG AARLRKVPLL
1261V
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1261 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.