LSBio
Human CNOT11 / C2orf29 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CNOT11 / C2orf29 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CNOT11 antibodies →
$153.00each
Catalog Number: LS-G90549-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C2ORF29(CNOT11) (NM_017546) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2orf29 | C40 | UPF0760 protein C2orf29
Origin Species
Human
NCBI GenBank
NM_017546
SwissProt
Q9UKZ1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPGGGASAAS GRLLTAAEQR GSREAAGSAS RSGFGGSGGG RGGASGPGSG SGGPGGPAGR
61MSLTPKELSS LLSIISEEAG GGSTFEGLST AFHHYFSKAD HFRLGSVLVM LLQQPDLLPS
121AAQRLTALYL LWEMYRTEPL AANPFAASFA HLLNPAPPAR GGQEPDRPPL SGFLPPITPP
181EKFFLSQLML APPRELFKKT PRQIALMDVG NMGQSVDISG LQLALAERQS ELPTQSKASF
241PSILSDPDPD SSNSGFDSSV ASQITEALVS GPKPPIESHF RPEFIRPPPP LHICEDELAW
301LNPTEPDHAI QWDKSMCVKN STGVEIKRIM AKAFKSPLSS PQQTQLLGEL EKDPKLVYHI
361GLTPAKLPDL VENNPLVAIE MLLKLMQSSQ ITEYFSVLVN MDMSLHSMEV VNRLTTAVDL
421PPEFIHLYIS NCISTCEQIK DKYMQNRLVR LVCVFLQSLI RNKIINVQDL FIEVQAFCIE
481FSRIREAAGL FRLLKTLDTG ETPSETEMSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

510 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.