Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CNOT11 / C2orf29 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112366-100
Catalog Number: LS-G112366
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C2ORF29(CNOT11) (NM_017546), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C2orf29 | C40 | UPF0760 protein C2orf29
- Origin Species
- Human
- NCBI GenBank
- NM_017546
- SwissProt
- Q9UKZ1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGGGASAAS GRLLTAAEQR GSREAAGSAS RSGFGGSGGG RGGASGPGSG SGGPGGPAGR61MSLTPKELSS LLSIISEEAG GGSTFEGLST AFHHYFSKAD HFRLGSVLVM LLQQPDLLPS121AAQRLTALYL LWEMYRTEPL AANPFAASFA HLLNPAPPAR GGQEPDRPPL SGFLPPITPP181EKFFLSQLML APPRELFKKT PRQIALMDVG NMGQSVDISG LQLALAERQS ELPTQSKASF241PSILSDPDPD SSNSGFDSSV ASQITEALVS GPKPPIESHF RPEFIRPPPP LHICEDELAW301LNPTEPDHAI QWDKSMCVKN STGVEIKRIM AKAFKSPLSS PQQTQLLGEL EKDPKLVYHI361GLTPAKLPDL VENNPLVAIE MLLKLMQSSQ ITEYFSVLVN MDMSLHSMEV VNRLTTAVDL421PPEFIHLYIS NCISTCEQIK DKYMQNRLVR LVCVFLQSLI RNKIINVQDL FIEVQAFCIE481FSRIREAAGL FRLLKTLDTG ETPSETEMSKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
510 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.