LSBio
Human CLIP2 / CYLN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CLIP2 / CYLN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CLIP2 antibodies →
$220.00each
Catalog Number: LS-G79828-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CYLN2(CLIP2) (NM_003388) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLIP | Cytoplasmic linker protein 2 | CYLN2 | Cytoplasmic linker 2 | Cytoplasmic linker protein 115 | KIAA0291 | WBSCR4 | WSCR4 | WBSCR3 | CLIP-115 | CLIP2 | WSCR3
Origin Species
Human
NCBI GenBank
NM_003388
SwissProt
Q9UDT6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQKPSGLKPP GRGGKHSSPM GRTSTGSASS SAAVAASSKE GSPLHKQSSG PSSSPAAAAA
61PEKPGPKAAE VGDDFLGDFV VGERVWVNGV KPGVVQYLGE TQFAPGQWAG VVLDDPVGKN
121DGAVGGVRYF ECPALQGIFT RPSKLTRQPT AEGSGSDAHS VESLTAQNLS LHSGTATPPL
181TSRVIPLRES VLNSSVKTGN ESGSNLSDSG SVKRGEKDLR LGDRVLVGGT KTGVVRYVGE
241TDFAKGEWCG VELDEPLGKN DGAVAGTRYF QCPPKFGLFA PIHKVIRIGF PSTSPAKAKK
301TKRMAMGVSA LTHSPSSSSI SSVSSVASSV GGRPSRSGLL TETSSRYARK ISGTTALQEA
361LKEKQQHIEQ LLAERDLERA EVAKATSHIC EVEKEIALLK AQHEQYVAEA EEKLQRARLL
421VESVRKEKVD LSNQLEEERR KVEDLQFRVE EESITKGDLE TQTQLEHARI GELEQSLLLE
481KAQAERLLRE LADNRLTTVA EKSRVLQLEE ELTLRRGEIE ELQQCLLHSG PPPPDHPDAA
541EILRLRERLL SASKEHQRES GVLRDKYEKA LKAYQAEVDK LRAANEKYAQ EVAGLKDKVQ
601QATSENMGLM DNWKSKLDSL ASDHQKSLED LKATLNSGPG AQQKEIGELK AVMEGIKMEH
661QLELGNLQAK HDLETAMHVK EKEALREKLQ EAQEELAGLQ RHWRAQLEVQ ASQHRLELQE
721AQDQRRDAEL RVHELEKLDV EYRGQAQAIE FLKEQISLAE KKMLDYERLQ RAEAQGKQEV
781ESLREKLLVA ENRLQAVEAL CSSQHTHMIE SNDISEETIR TKETVEGLQD KLNKRDKEVT
841ALTSQTEMLR AQVSALESKC KSGEKKVDAL LKEKRRLEAE LETVSRKTHD ASGQLVLISQ
901ELLRKERSLN ELRVLLLEAN RHSPGPERDL SREVHKAEWR IKEQKLKDDI RGLREKLTGL
961DKEKSLSDQR RYSLIDPSSA PELLRLQHQL MSTEDALRDA LDQAQQVEKL MEAMRSCPDK
1021AQTIGNSGSA NGIHQQDKAQ KQEDKH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1046 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.