Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CLIP2 / CYLN2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117336-100
Catalog Number: LS-G117336
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CYLN2(CLIP2) (NM_003388), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CLIP | Cytoplasmic linker protein 2 | CYLN2 | Cytoplasmic linker 2 | Cytoplasmic linker protein 115 | KIAA0291 | WBSCR4 | WSCR4 | WBSCR3 | CLIP-115 | CLIP2 | WSCR3
- Origin Species
- Human
- NCBI GenBank
- NM_003388
- SwissProt
- Q9UDT6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 115.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQKPSGLKPP GRGGKHSSPM GRTSTGSASS SAAVAASSKE GSPLHKQSSG PSSSPAAAAA61PEKPGPKAAE VGDDFLGDFV VGERVWVNGV KPGVVQYLGE TQFAPGQWAG VVLDDPVGKN121DGAVGGVRYF ECPALQGIFT RPSKLTRQPT AEGSGSDAHS VESLTAQNLS LHSGTATPPL181TSRVIPLRES VLNSSVKTGN ESGSNLSDSG SVKRGEKDLR LGDRVLVGGT KTGVVRYVGE241TDFAKGEWCG VELDEPLGKN DGAVAGTRYF QCPPKFGLFA PIHKVIRIGF PSTSPAKAKK301TKRMAMGVSA LTHSPSSSSI SSVSSVASSV GGRPSRSGLL TETSSRYARK ISGTTALQEA361LKEKQQHIEQ LLAERDLERA EVAKATSHIC EVEKEIALLK AQHEQYVAEA EEKLQRARLL421VESVRKEKVD LSNQLEEERR KVEDLQFRVE EESITKGDLE TQTQLEHARI GELEQSLLLE481KAQAERLLRE LADNRLTTVA EKSRVLQLEE ELTLRRGEIE ELQQCLLHSG PPPPDHPDAA541EILRLRERLL SASKEHQRES GVLRDKYEKA LKAYQAEVDK LRAANEKYAQ EVAGLKDKVQ601QATSENMGLM DNWKSKLDSL ASDHQKSLED LKATLNSGPG AQQKEIGELK AVMEGIKMEH661QLELGNLQAK HDLETAMHVK EKEALREKLQ EAQEELAGLQ RHWRAQLEVQ ASQHRLELQE721AQDQRRDAEL RVHELEKLDV EYRGQAQAIE FLKEQISLAE KKMLDYERLQ RAEAQGKQEV781ESLREKLLVA ENRLQAVEAL CSSQHTHMIE SNDISEETIR TKETVEGLQD KLNKRDKEVT841ALTSQTEMLR AQVSALESKC KSGEKKVDAL LKEKRRLEAE LETVSRKTHD ASGQLVLISQ901ELLRKERSLN ELRVLLLEAN RHSPGPERDL SREVHKAEWR IKEQKLKDDI RGLREKLTGL961DKEKSLSDQR RYSLIDPSSA PELLRLQHQL MSTEDALRDA LDQAQQVEKL MEAMRSCPDK1021AQTIGNSGSA NGIHQQDKAQ KQEDKHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1046 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.