CLIP1 / CLIP-170 Antibody
Catalog Number: LS-C747597
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CLIP1 / CLIP-170 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G78611-20
Catalog Number: LS-G78611
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CLIP170(CLIP1) (NM_198240) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CLIP | CLIP-170 | CLIP1 | CYLN1 | Cytoplasmic linker protein 1 | Restin | CLIP170 | RSN
- Origin Species
- Human
- NCBI GenBank
- NM_002956
- SwissProt
- P30622
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 156.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSMLKPSGLK APTKILKPGS TALKTPTAVV APVEKTISSE KASSTPSSET QEEFVDDFRV61GERVWVNGNK PGFIQFLGET QFAPGQWAGI VLDEPIGKND GSVAGVRYFQ CEPLKGIFTR121PSKLTRKVQA EDEANGLQTT PASRATSPLC TSTASMVSSS PSTPSNIPQK PSQPAAKEPS181ATPPISNLTK TASESISNLS EAGSIKKGER ELKIGDRVLV GGTKAGVVRF LGETDFAKGE241WCGVELDEPL GKNDGAVAGT RYFQCQPKYG LFAPVHKVTK IGFPSTTPAK AKANAVRRVM301ATTSASLKRS PSASSLSSMS SVASSVSSRP SRTGLLTETS SRYARKISGT TALQEALKEK361QQHIEQLLAE RDLERAEVAK ATSHVGEIEQ ELALARDGHD QHVLELEAKM DQLRTMVEAA421DREKVELLNQ LEEEKRKVED LQFRVEEESI TKGDLEVATV SEKSRIMELE KDLALRVQEV481AELRRRLESN KPAGDVDMSL SLLQEISSLQ EKLEVTRTDH QREITSLKEH FGAREETHQK541EIKALYTATE KLSKENESLK SKLEHANKEN SDVIALWKSK LETAIASHQQ AMEELKVSFS601KGLGTETAEF AELKTQIEKM RLDYQHEIEN LQNQQDSERA AHAKEMEALR AKLMKVIKEK661ENSLEAIRSK LDKAEDQHLV EMEDTLNKLQ EAEIKVKELE VLQAKCNEQT KVIDNFTSQL721KATEEKLLDL DALRKASSEG KSEMKKLRQQ LEAAEKQIKH LEIEKNAESS KASSITRELQ781GRELKLTNLQ ENLSEVSQVK ETLEKELQIL KEKFAEASEE AVSVQRSMQE TVNKLHQKEE841QFNMLSSDLE KLRENLADME AKFREKDERE EQLIKAKEKL ENDIAEIMKM SGDNSSQLTK901MNDELRLKER DVEELQLKLT KANENASFLQ KSIEDMTVKA EQSQQEAAKK HEEEKKELER961KLSDLEKKME TSHNQCQELK ARYERATSET KTKHEEILQN LQKTLLDTED KLKGAREENS1021GLLQELEELR KQADKAKAAQ TAEDAMQIME QMTKEKTETL ASLEDTKQTN AKLQNELDTL1081KENNLKNVEE LNKSKELLTV ENQKMEEFRK EIETLKQAAA QKSQQLSALQ EENVKLAEEL1141GRSRDEVTSH QKLEEERSVL NNQLLEMKKR ESKFIKDADE EKASLQKSIS ITSALLTEKD1201AELEKLRNEV TVLRGENASA KSLHSVVQTL ESDKVKLELK VKNLELQLKE NKRQLSSSSG1261NTDTQADEDE RAQESQIDFL NSVIVDLQRK NQDLKMKVEM MSEAALNGNG DDLNNYDSDD1321QEKQSKKKPR LFCDICDCFD LHDTEDCPTQ AQMSEDPPHS THHGSRGEER PYCEICEMFG1381HWATNCNDDE TFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1392 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
