Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CLIP1 / CLIP-170 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103727-100
Catalog Number: LS-G103727
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CLIP170(CLIP1) (NM_198240), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CLIP | CLIP-170 | CLIP1 | CYLN1 | Cytoplasmic linker protein 1 | Restin | CLIP170 | RSN
- Origin Species
- Human
- NCBI GenBank
- NM_002956
- SwissProt
- P30622
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 156.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSMLKPSGLK APTKILKPGS TALKTPTAVV APVEKTISSE KASSTPSSET QEEFVDDFRV61GERVWVNGNK PGFIQFLGET QFAPGQWAGI VLDEPIGKND GSVAGVRYFQ CEPLKGIFTR121PSKLTRKVQA EDEANGLQTT PASRATSPLC TSTASMVSSS PSTPSNIPQK PSQPAAKEPS181ATPPISNLTK TASESISNLS EAGSIKKGER ELKIGDRVLV GGTKAGVVRF LGETDFAKGE241WCGVELDEPL GKNDGAVAGT RYFQCQPKYG LFAPVHKVTK IGFPSTTPAK AKANAVRRVM301ATTSASLKRS PSASSLSSMS SVASSVSSRP SRTGLLTETS SRYARKISGT TALQEALKEK361QQHIEQLLAE RDLERAEVAK ATSHVGEIEQ ELALARDGHD QHVLELEAKM DQLRTMVEAA421DREKVELLNQ LEEEKRKVED LQFRVEEESI TKGDLEVATV SEKSRIMELE KDLALRVQEV481AELRRRLESN KPAGDVDMSL SLLQEISSLQ EKLEVTRTDH QREITSLKEH FGAREETHQK541EIKALYTATE KLSKENESLK SKLEHANKEN SDVIALWKSK LETAIASHQQ AMEELKVSFS601KGLGTETAEF AELKTQIEKM RLDYQHEIEN LQNQQDSERA AHAKEMEALR AKLMKVIKEK661ENSLEAIRSK LDKAEDQHLV EMEDTLNKLQ EAEIKVKELE VLQAKCNEQT KVIDNFTSQL721KATEEKLLDL DALRKASSEG KSEMKKLRQQ LEAAEKQIKH LEIEKNAESS KASSITRELQ781GRELKLTNLQ ENLSEVSQVK ETLEKELQIL KEKFAEASEE AVSVQRSMQE TVNKLHQKEE841QFNMLSSDLE KLRENLADME AKFREKDERE EQLIKAKEKL ENDIAEIMKM SGDNSSQLTK901MNDELRLKER DVEELQLKLT KANENASFLQ KSIEDMTVKA EQSQQEAAKK HEEEKKELER961KLSDLEKKME TSHNQCQELK ARYERATSET KTKHEEILQN LQKTLLDTED KLKGAREENS1021GLLQELEELR KQADKAKAAQ TAEDAMQIME QMTKEKTETL ASLEDTKQTN AKLQNELDTL1081KENNLKNVEE LNKSKELLTV ENQKMEEFRK EIETLKQAAA QKSQQLSALQ EENVKLAEEL1141GRSRDEVTSH QKLEEERSVL NNQLLEMKKR ESKFIKDADE EKASLQKSIS ITSALLTEKD1201AELEKLRNEV TVLRGENASA KSLHSVVQTL ESDKVKLELK VKNLELQLKE NKRQLSSSSG1261NTDTQADEDE RAQESQIDFL NSVIVDLQRK NQDLKMKVEM MSEAALNGNG DDLNNYDSDD1321QEKQSKKKPR LFCDICDCFD LHDTEDCPTQ AQMSEDPPHS THHGSRGEER PYCEICEMFG1381HWATNCNDDE TFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1392 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.