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Human ClC-5 / CLCN5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ClC-5 / CLCN5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CLCN5 antibodies →
$220.00each
Catalog Number: LS-G75164-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Chloride Channel 5(CLCN5) (NM_000084) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLCN5 | DENTS | CLC5 | HCIC-K2 | Nephrolithiasis 1 (X-linked) | Nephrolithiasis 2 | X-linked | NPHL1 | NPHL2 | XLRH | XRN | Chloride channel 5 | Chloride channel protein 5 | Chloride transporter ClC-5 | ClC-5 | CLCK2 | HClC-K2
Origin Species
Human
NCBI GenBank
NM_000084
SwissProt
P51795

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDIPATAMDF SMRDDVPPLD REVGEDKSYN GGGIGSSNRI MDFLEEPIPG VGTYDDFNTI
61DWVREKSRDR DRHREITNKS KESTWALIHS VSDAFSGWLL MLLIGLLSGS LAGLIDISAH
121WMTDLKEGIC TGGFWFNHEH CCWNSEHVTF EERDKCPEWN SWSQLIISTD EGAFAYIVNY
181FMYVLWALLF AFLAVSLVKV FAPYACGSGI PEIKTILSGF IIRGYLGKWT LVIKTITLVL
241AVSSGLSLGK EGPLVHVACC CGNILCHCFN KYRKNEAKRR EVLSAAAAAG VSVAFGAPIG
301GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN SRLVLFYVEF HTPWHLFELV
361PFILLGIFGG LWGALFIRTN IAWCRKRKTT QLGKYPVIEV LVVTAITAIL AFPNEYTRMS
421TSELISELFN DCGLLDSSKL CDYENRFNTS KGGELPDRPA GVGVYSAMWQ LALTLILKIV
481ITIFTFGMKI PSGLFIPSMA VGAIAGRLLG VGMEQLAYYH QEWTVFNSWC SQGADCITPG
541LYAMVGAAAC LGGVTRMTVS LVVIMFELTG GLEYIVPLMA AAMTSKWVAD ALGREGIYDA
601HIRLNGYPFL EAKEEFAHKT LAMDVMKPRR NDPLLTVLTQ DSMTVEDVET IISETTYSGF
661PVVVSRESQR LVGFVLRRDL IISIENARKK QDGVVSTSII YFTEHSPPLP PYTPPTLKLR
721NILDLSPFTV TDLTPMEIVV DIFRKLGLRQ CLVTHNGRLL GIITKKDVLK HIAQMANQDP
781DSILFN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

786 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.