Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ClC-5 / CLCN5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G98910-100
Catalog Number: LS-G98910
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Chloride Channel 5(CLCN5) (NM_000084), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CLCN5 | DENTS | CLC5 | HCIC-K2 | Nephrolithiasis 1 (X-linked) | Nephrolithiasis 2 | X-linked | NPHL1 | NPHL2 | XLRH | XRN | Chloride channel 5 | Chloride channel protein 5 | Chloride transporter ClC-5 | ClC-5 | CLCK2 | HClC-K2
- NCBI GenBank
- NM_000084
- SwissProt
- P51795
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 83 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDIPATAMDF SMRDDVPPLD REVGEDKSYN GGGIGSSNRI MDFLEEPIPG VGTYDDFNTI61DWVREKSRDR DRHREITNKS KESTWALIHS VSDAFSGWLL MLLIGLLSGS LAGLIDISAH121WMTDLKEGIC TGGFWFNHEH CCWNSEHVTF EERDKCPEWN SWSQLIISTD EGAFAYIVNY181FMYVLWALLF AFLAVSLVKV FAPYACGSGI PEIKTILSGF IIRGYLGKWT LVIKTITLVL241AVSSGLSLGK EGPLVHVACC CGNILCHCFN KYRKNEAKRR EVLSAAAAAG VSVAFGAPIG301GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN SRLVLFYVEF HTPWHLFELV361PFILLGIFGG LWGALFIRTN IAWCRKRKTT QLGKYPVIEV LVVTAITAIL AFPNEYTRMS421TSELISELFN DCGLLDSSKL CDYENRFNTS KGGELPDRPA GVGVYSAMWQ LALTLILKIV481ITIFTFGMKI PSGLFIPSMA VGAIAGRLLG VGMEQLAYYH QEWTVFNSWC SQGADCITPG541LYAMVGAAAC LGGVTRMTVS LVVIMFELTG GLEYIVPLMA AAMTSKWVAD ALGREGIYDA601HIRLNGYPFL EAKEEFAHKT LAMDVMKPRR NDPLLTVLTQ DSMTVEDVET IISETTYSGF661PVVVSRESQR LVGFVLRRDL IISIENARKK QDGVVSTSII YFTEHSPPLP PYTPPTLKLR721NILDLSPFTV TDLTPMEIVV DIFRKLGLRQ CLVTHNGRLL GIITKKDVLK HIAQMANQDP781DSILFNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
786 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.