LSBio
Human CGN / Cingulin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CGN / Cingulin Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CGN antibodies →
$220.00each
Catalog Number: LS-G82732-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Cingulin(CGN) (NM_020770) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CGN | Cingulin | KIAA1319
Origin Species
Human
NCBI GenBank
AB037740
SwissProt
Q9P2M7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
136.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEQAPNMAEP RGPVDHGVQI RFITEPVSGA EMGTLRRGGR RPAKDARAST YGVAVRVQGI
61AGQPFVVLNS GEKGGDSFGV QIKGANDQGA SGALSSDLEL PENPYSQVKG FPAPSQSSTS
121DEEPGAYWNG KLLRSHSQAS LAGPGPVDPS NRSNSMLELA PKVASPGSTI DTAPLSSVDS
181LINKFDSQLG GQARGRTGRR TRMLPPEQRK RSKSLDSRLP RDTFEERERQ STNHWTSSTK
241YDNHVGTSKQ PAQSQNLSPL SGFSRSRQTQ DWVLQSFEEP RRSAQDPTML QFKSTPDLLR
301DQQEAAPPGS VDHMKATIYG ILREGSSESE TSVRRKVSLV LEKMQPLVMV SSGSTKAVAG
361QGELTRKVEE LQRKLDEEVK KRQKLEPSQV GLERQLEEKT EECSRLQELL ERRKGEAQQS
421NKELQNMKRL LDQGEDLRHG LETQVMELQN KLKHVQGPEP AKEVLLKDLL ETRELLEEVL
481EGKQRVEEQL RLRERELTAL KGALKEEVAS RDQEVEHVRQ QYQRDTEQLR RSMQDATQDH
541AVLEAERQKM SALVRGLQRE LEETSEETGH WQSMFQKNKE DLRATKQELL QLRMEKEEME
601EELGEKIEVL QRELEQARAS AGDTRQVEVL KKELLRTQEE LKELQAERQS QEVAGRHRDR
661ELEKQLAVLR VEADRGRELE EQNLQLQKTL QQLRQDCEEA SKAKMVAEAE ATVLGQRRAA
721VETTLRETQE ENDEFRRRIL GLEQQLKETR GLVDGGEAVE ARLRDKLQRL EAEKQQLEEA
781LNASQEEEGS LAAAKRALEA RLEEAQRGLA RLGQEQQTLN RALEEEGKQR EVLRRGKAEL
841EEQKRLLDRT VDRLNKELEK IGEDSKQALQ QLQAQLEDYK EKARREVADA QRQAKDWASE
901AEKTSGGLSR LQDEIQRLRQ ALQASQAERD TARLDKELLA QRLQGLEQEA ENKKRSQDDR
961ARQLKGLEEK VSRLETELDE EKNTVELLTD RVNRGRDQVD QLRTELMQER SARQDLECDK
1021ISLERQNKDL KTRLASSEGF QKPSASLSQL ESQNQLLQER LQAEEREKTV LQSTNRKLER
1081KVKELSIQIE DERQHVNDQK DQLSLRVKAL KRQVDEAEEE IERLDGLRKK AQREVEEQHE
1141VNEQLQARIK SLEKDSWRKA SRSAAESALK NEGLSSDEEF DSVYDPSSIA SLLTESNLQT
1201SSC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1203 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.