Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CGN / Cingulin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111010-100
Catalog Number: LS-G111010
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cingulin(CGN) (NM_020770), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CGN | Cingulin | KIAA1319
- Origin Species
- Human
- NCBI GenBank
- AB037740
- SwissProt
- Q9P2M7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 136.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEQAPNMAEP RGPVDHGVQI RFITEPVSGA EMGTLRRGGR RPAKDARAST YGVAVRVQGI61AGQPFVVLNS GEKGGDSFGV QIKGANDQGA SGALSSDLEL PENPYSQVKG FPAPSQSSTS121DEEPGAYWNG KLLRSHSQAS LAGPGPVDPS NRSNSMLELA PKVASPGSTI DTAPLSSVDS181LINKFDSQLG GQARGRTGRR TRMLPPEQRK RSKSLDSRLP RDTFEERERQ STNHWTSSTK241YDNHVGTSKQ PAQSQNLSPL SGFSRSRQTQ DWVLQSFEEP RRSAQDPTML QFKSTPDLLR301DQQEAAPPGS VDHMKATIYG ILREGSSESE TSVRRKVSLV LEKMQPLVMV SSGSTKAVAG361QGELTRKVEE LQRKLDEEVK KRQKLEPSQV GLERQLEEKT EECSRLQELL ERRKGEAQQS421NKELQNMKRL LDQGEDLRHG LETQVMELQN KLKHVQGPEP AKEVLLKDLL ETRELLEEVL481EGKQRVEEQL RLRERELTAL KGALKEEVAS RDQEVEHVRQ QYQRDTEQLR RSMQDATQDH541AVLEAERQKM SALVRGLQRE LEETSEETGH WQSMFQKNKE DLRATKQELL QLRMEKEEME601EELGEKIEVL QRELEQARAS AGDTRQVEVL KKELLRTQEE LKELQAERQS QEVAGRHRDR661ELEKQLAVLR VEADRGRELE EQNLQLQKTL QQLRQDCEEA SKAKMVAEAE ATVLGQRRAA721VETTLRETQE ENDEFRRRIL GLEQQLKETR GLVDGGEAVE ARLRDKLQRL EAEKQQLEEA781LNASQEEEGS LAAAKRALEA RLEEAQRGLA RLGQEQQTLN RALEEEGKQR EVLRRGKAEL841EEQKRLLDRT VDRLNKELEK IGEDSKQALQ QLQAQLEDYK EKARREVADA QRQAKDWASE901AEKTSGGLSR LQDEIQRLRQ ALQASQAERD TARLDKELLA QRLQGLEQEA ENKKRSQDDR961ARQLKGLEEK VSRLETELDE EKNTVELLTD RVNRGRDQVD QLRTELMQER SARQDLECDK1021ISLERQNKDL KTRLASSEGF QKPSASLSQL ESQNQLLQER LQAEEREKTV LQSTNRKLER1081KVKELSIQIE DERQHVNDQK DQLSLRVKAL KRQVDEAEEE IERLDGLRKK AQREVEEQHE1141VNEQLQARIK SLEKDSWRKA SRSAAESALK NEGLSSDEEF DSVYDPSSIA SLLTESNLQT1201SSCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1203 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.