LSBio
Human CFAP74 / KIAA1751 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CFAP74 / KIAA1751 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CFAP74 antibodies →
$220.00each
Catalog Number: LS-G95128-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1751(CFAP74) (NM_001080484) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1751 | RP1-140A9.6
Origin Species
Human
NCBI GenBank
NM_001080484
SwissProt
Q9C0B2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MEDDGSLLPE DELLADALLL EDERDELEDP EFDIKCLLQE AEDDVDPGHS SSVKELDTDA
61DKLKKKTAED RTQAFHLRQN LSALDKMHEE QELFTEKMRG ELRACRQRRD LIDKQQEAVA
121AEIATEEEAG NMAAVGRLQA VSRRLFAELE NERDLQSRTE AVLKESENTM WHIEIQEGRL
181EAFRTADREE VEATGRRLQV RAAEQLCREQ EALGKVERNR LLRIRKSLNT QKELGLRHQK
241LLEDARKNHK VAVRFLKASL GRIREQEKKE EMECHEYMRR RMDAVVALKG SISANRDTLR
301KFQAWDRAKA ELAEQRVQAE KKAILAQGRD AFRHLVHQRR RQELEAQKRA FEEEQKLRKQ
361EIISRILKEE AEEEKRKKQH PPTSARHRLT LRDKTWNYIS DFCKKTTVPT NTYTLDYEAA
421AGPGPSRLLE VVSSELIQGD PGASSEEETL AEPEISGLWN EDYKPYQVPK EDVDRKPVGG
481TKMDKDILER TVERLRSRVV HKQVVWGREF QGRPFNSKPE LLHFQDFDIG KVYKKKITLV
541NTTYTINYCK LVGVEEHLRD FIHVDFDPPG PLSAGMSCEV LVTFKPMINK DLEGNISFLA
601QTGEFSVPLK CSTKKCSLSL DKELIDFGSY VVGETTSRTI TLTNVGGLGT TFKFLPASEP
661CEMDDSQSAL KLSSLLTYED KSLYDKAATS FSEQQLEGTE SSQADMQSRK ELEKLDKEQE
721EEQPAGEPGC QAKPGWAGGE GLRAAFQGAE GPRGGLESRE FS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

762 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.