Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CFAP74 / KIAA1751 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119642-100
Catalog Number: LS-G119642
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1751(CFAP74) (NM_001080484), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1751 | RP1-140A9.6
- Origin Species
- Human
- NCBI GenBank
- NM_001080484
- SwissProt
- Q9C0B2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEDDGSLLPE DELLADALLL EDERDELEDP EFDIKCLLQE AEDDVDPGHS SSVKELDTDA61DKLKKKTAED RTQAFHLRQN LSALDKMHEE QELFTEKMRG ELRACRQRRD LIDKQQEAVA121AEIATEEEAG NMAAVGRLQA VSRRLFAELE NERDLQSRTE AVLKESENTM WHIEIQEGRL181EAFRTADREE VEATGRRLQV RAAEQLCREQ EALGKVERNR LLRIRKSLNT QKELGLRHQK241LLEDARKNHK VAVRFLKASL GRIREQEKKE EMECHEYMRR RMDAVVALKG SISANRDTLR301KFQAWDRAKA ELAEQRVQAE KKAILAQGRD AFRHLVHQRR RQELEAQKRA FEEEQKLRKQ361EIISRILKEE AEEEKRKKQH PPTSARHRLT LRDKTWNYIS DFCKKTTVPT NTYTLDYEAA421AGPGPSRLLE VVSSELIQGD PGASSEEETL AEPEISGLWN EDYKPYQVPK EDVDRKPVGG481TKMDKDILER TVERLRSRVV HKQVVWGREF QGRPFNSKPE LLHFQDFDIG KVYKKKITLV541NTTYTINYCK LVGVEEHLRD FIHVDFDPPG PLSAGMSCEV LVTFKPMINK DLEGNISFLA601QTGEFSVPLK CSTKKCSLSL DKELIDFGSY VVGETTSRTI TLTNVGGLGT TFKFLPASEP661CEMDDSQSAL KLSSLLTYED KSLYDKAATS FSEQQLEGTE SSQADMQSRK ELEKLDKEQE721EEQPAGEPGC QAKPGWAGGE GLRAAFQGAE GPRGGLESRE FSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
762 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.