Human CFAP70 / TTC18 Protein (Recombinant Myc-DDK (Flag))
Catalog Number: LS-G73652-20
20 µg / $1130.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CFAP70 / TTC18 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G95811-20
Catalog Number: LS-G95811
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TTC18(CFAP70) (NM_145170) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_145170
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 125.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEQVPSAGRL VQITVTEGYD LKGFKGDTPV TFIRAEFNQV VLGDSAKITV SPEGSAKYNF61TSSFEFNPEG GITSDDLAHK PVFLTVTEVL PKEKKQKEEK TLILGQAVVD LLPLLEGQSS121FQTTVPLHPV QGSPLETPRS SAKQCSLEVK VLVAEPLLTT AQISGGNLLK VTLEAAYSVP181ESFIPTGPGQ NYMVGLQVPS LGEKDYPILF KNGTLKLGGE REPVPRPKKW PIANILAPGA241NNIPDAFIVG GPYEEEEGEL NHPEDSEFRN QAECIKKRII WDLESRCYLD PSAVVSFQKR301IADCRLWPVE ITRVPLVTIP KGKAGKTEKT DEEAQLSFHG VAYVNMVPLL YPGVKRIRGA361FHVYPYLDSV VHEKTKCLLS LFRDIGHHLI HNNKIGGINS LLSKQAVSKN LKEDKPVKEK421DIDGRPRPGD VQAPSIKSQS SDTPLEGEPP LSHNPEGQQY VEAGTYIVLE IQLDKALVPK481RMPEELARRV KEMIPPRPPL TRRTGGAQKA MSDYHIQIKN ISRAILDEYY RMFGKQVAKL541ESDMDSETLE EQKCQLSYEL NCSGKYFAFK EQLKHAVVKI VRDKYLKTTS FESQEELQTF601ISELYVFLVD QMHVALNQTM PDDVQGTVAT IYTSSEQLQL FAFEAEVNEN FEMAAAYYKE661RLVREPQNLD HWLDYGAFCL LTEDNIKAQE CFQKALSLNQ SHIHSLLLCG VLAVLLENYE721QAEIFFEDAT CLEPTNVVAW TLLGLYYEIQ NNDIRMEMAF HEASKQLQAR MLQAQVTKQK781STGVEDTEER GKRESSLGPW GITNGSATAI KVEAPAGPGA ALSILDKFLE ESSKLQSDSQ841EPILTTQTWD PSISQKPSNT FIKEIPTKKE ASKCQDSSAL LHPGLHYGVS QTTTIFMETI901HFLMKVKAVQ YVHRVLAHEL LCPQGGPSCE YYLVLAQTHI LKKNFAKAEE YLQQAAQMDY961LNPNVWGLKG HLYFLSGNHS EAKACYERTI SFVVDASEMH FIFLRLGLIY LEEKEYEKAK1021KTYMQACKRS PSCLTWLGLG IACYRLEELT EAEDALSEAN ALNNYNAEVW AYLALVCLKV1081GRQLEAEQAY KYMIKLKLKD EALLAEIHTL QETVGFGNPS FTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1121 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
