Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CFAP70 / TTC18 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106747-100
Catalog Number: LS-G106747
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC18(CFAP70) (NM_145170), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_145170
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 125.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEQVPSAGRL VQITVTEGYD LKGFKGDTPV TFIRAEFNQV VLGDSAKITV SPEGSAKYNF61TSSFEFNPEG GITSDDLAHK PVFLTVTEVL PKEKKQKEEK TLILGQAVVD LLPLLEGQSS121FQTTVPLHPV QGSPLETPRS SAKQCSLEVK VLVAEPLLTT AQISGGNLLK VTLEAAYSVP181ESFIPTGPGQ NYMVGLQVPS LGEKDYPILF KNGTLKLGGE REPVPRPKKW PIANILAPGA241NNIPDAFIVG GPYEEEEGEL NHPEDSEFRN QAECIKKRII WDLESRCYLD PSAVVSFQKR301IADCRLWPVE ITRVPLVTIP KGKAGKTEKT DEEAQLSFHG VAYVNMVPLL YPGVKRIRGA361FHVYPYLDSV VHEKTKCLLS LFRDIGHHLI HNNKIGGINS LLSKQAVSKN LKEDKPVKEK421DIDGRPRPGD VQAPSIKSQS SDTPLEGEPP LSHNPEGQQY VEAGTYIVLE IQLDKALVPK481RMPEELARRV KEMIPPRPPL TRRTGGAQKA MSDYHIQIKN ISRAILDEYY RMFGKQVAKL541ESDMDSETLE EQKCQLSYEL NCSGKYFAFK EQLKHAVVKI VRDKYLKTTS FESQEELQTF601ISELYVFLVD QMHVALNQTM PDDVQGTVAT IYTSSEQLQL FAFEAEVNEN FEMAAAYYKE661RLVREPQNLD HWLDYGAFCL LTEDNIKAQE CFQKALSLNQ SHIHSLLLCG VLAVLLENYE721QAEIFFEDAT CLEPTNVVAW TLLGLYYEIQ NNDIRMEMAF HEASKQLQAR MLQAQVTKQK781STGVEDTEER GKRESSLGPW GITNGSATAI KVEAPAGPGA ALSILDKFLE ESSKLQSDSQ841EPILTTQTWD PSISQKPSNT FIKEIPTKKE ASKCQDSSAL LHPGLHYGVS QTTTIFMETI901HFLMKVKAVQ YVHRVLAHEL LCPQGGPSCE YYLVLAQTHI LKKNFAKAEE YLQQAAQMDY961LNPNVWGLKG HLYFLSGNHS EAKACYERTI SFVVDASEMH FIFLRLGLIY LEEKEYEKAK1021KTYMQACKRS PSCLTWLGLG IACYRLEELT EAEDALSEAN ALNNYNAEVW AYLALVCLKV1081GRQLEAEQAY KYMIKLKLKD EALLAEIHTL QETVGFGNPS FTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1121 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.