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Human CEP95 / CCDC45 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CEP95 / CCDC45 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CEP95 antibodies →
$153.00each
Catalog Number: LS-G82308-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CEP95 (NM_138363) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Centrosomal protein 95kDa | Centrosomal protein of 95 kDa | CEP95 | CCDC45 | CEP45
Origin Species
Human
NCBI GenBank
NM_138363
SwissProt
Q96GE4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGSDAEWVT IANNLLFKCH IHLRIHELQD CDANVFIALY QSILGEKVPD LIVIPRSQED
61DAHNVQAVID SLALDYLQVS LSHITGENIV KGDKESIKNL LEIFDGLLEY LTERISETSH
121EKSETEQYFK ESDRGERLEE PESTKESKSS WKRVSFGRCS LSSEMLGPSW DGDEAESTGE
181IIRLGDTAHT FSLRSNGAQC PNEMLSKKAL ASPSSKSHED MLYPPSVLSK SRTSFVEDTE
241TLSVSGIPNA RKLGEPIRAA IPLHPPYHPS EPRAPCPIGK EYLHSSHCSP AVNSTGEHTE
301FSGDLDDGLF LISKLPKGSK WEVYPAQVQG PRTRKPPKGK RNENRATASS CNSPFPQRPR
361KRLTEQELHD VSEKLSQRLS ELDWMLKSAL GDRIKEKTDH KEENTGNEEV EDGTEETLSQ
421HSDGIVEYGP KKSRPGLSMR RKPPYRSHSL SPSPVNKHKQ FHLERKRQRK PRETDVRQFQ
481AQAFTEAFER ELRRHKVQEN IGPLRIHEKE EETEKIYRGE AVRKGTPECS QPWKIYSRKT
541TTQSLRGGLP KPNKAVPMKV SEHSLLPLML EQFPFLYVSG PTLSKMWKQQ IAQVEQLKKE
601ACRENRSKKK LQDEIEEALR RHDLLTTLVK KEYEHNKRLQ DFKDCIRRQR LTQSKIKENR
661QQIVRARKYY DDYRVQLCAK MMRMRTREEM IFKKLFEEGL NIQKQRLRDL RNYAKEKRDE
721QRRRHQDELD SMENYYKDQF SLLAEAISQE HQELKAREKS QAQTLHKVKR ELRSKMEKEI
781QQLQDMITQN DDDVFFRELE AERFRSRLQL ASFQYSKSPS L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

821 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.