Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CEP95 / CCDC45 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107344-100
Catalog Number: LS-G107344
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEP95 (NM_138363), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Centrosomal protein 95kDa | Centrosomal protein of 95 kDa | CEP95 | CCDC45 | CEP45
- Origin Species
- Human
- NCBI GenBank
- NM_138363
- SwissProt
- Q96GE4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 95.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGSDAEWVT IANNLLFKCH IHLRIHELQD CDANVFIALY QSILGEKVPD LIVIPRSQED61DAHNVQAVID SLALDYLQVS LSHITGENIV KGDKESIKNL LEIFDGLLEY LTERISETSH121EKSETEQYFK ESDRGERLEE PESTKESKSS WKRVSFGRCS LSSEMLGPSW DGDEAESTGE181IIRLGDTAHT FSLRSNGAQC PNEMLSKKAL ASPSSKSHED MLYPPSVLSK SRTSFVEDTE241TLSVSGIPNA RKLGEPIRAA IPLHPPYHPS EPRAPCPIGK EYLHSSHCSP AVNSTGEHTE301FSGDLDDGLF LISKLPKGSK WEVYPAQVQG PRTRKPPKGK RNENRATASS CNSPFPQRPR361KRLTEQELHD VSEKLSQRLS ELDWMLKSAL GDRIKEKTDH KEENTGNEEV EDGTEETLSQ421HSDGIVEYGP KKSRPGLSMR RKPPYRSHSL SPSPVNKHKQ FHLERKRQRK PRETDVRQFQ481AQAFTEAFER ELRRHKVQEN IGPLRIHEKE EETEKIYRGE AVRKGTPECS QPWKIYSRKT541TTQSLRGGLP KPNKAVPMKV SEHSLLPLML EQFPFLYVSG PTLSKMWKQQ IAQVEQLKKE601ACRENRSKKK LQDEIEEALR RHDLLTTLVK KEYEHNKRLQ DFKDCIRRQR LTQSKIKENR661QQIVRARKYY DDYRVQLCAK MMRMRTREEM IFKKLFEEGL NIQKQRLRDL RNYAKEKRDE721QRRRHQDELD SMENYYKDQF SLLAEAISQE HQELKAREKS QAQTLHKVKR ELRSKMEKEI781QQLQDMITQN DDDVFFRELE AERFRSRLQL ASFQYSKSPS LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
821 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.