LSBio
Human CEP78 / IP63 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CEP78 / IP63 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CEP78 antibodies →
$220.00each
Catalog Number: LS-G82368-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CEP78 (NM_032171) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C9orf81 | CEP78 | Centrosomal protein of 78 kDa | Centrosomal protein 78kDa | IP63
Origin Species
Human
NCBI GenBank
AK022705
SwissProt
Q5JTW2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIDSVKLRRD SAADFFSHYE YLCALQNSVP LPAVRACLRE GVLDFNADRL RGVDWAPLLS
61TLKINKDLPL VSIKSFFQPW LGDTGSDMNK FCRSRVPAIR YKDVTFQLCK ALKGCLSISS
121VLKNLELNGL ILRERDLTIL AKGLNKSASL VHLSLANCPI GDGGLEIICQ GIKSSITLKT
181VNFTGCNLTW QGADHMAKIL KYQTMRRHEE TWAESLRYRR PDLDCMAGLR RITLNCNTLI
241GDLGACAFAD SLSEDLWLRA LDLQQCGLTN EGAKALLEAL ETNTTLVVLD IRKNPLIDHS
301MMKAVIKKVL QNGRSAKSEY QWITSPSVKE PSKTAKQKRR TIILGSGHKG KATIRIVGLA
361TKKPVSSGRK HSLGKEYYAP APLPPGVSGF LPWRTAERAK RHRGFPLIKT RDICNQLQQP
421GFPVTVTVES PSSSEVEEVD DSSESVHEVP EKTSIEQEAL QEKLEECLKQ LKEERVIRLK
481VDKRVSELEH ENAQLRNINF SLSEALHAQS LTNMILDDEG VLGSIENSFQ KFHAFLDLLK
541DAGLGQLATM AGIDQSDFQL LGHPQMTSTV SNPPKEEKKA LEDEKPEPKQ NALGQMQNIQ
601FQKITGDARI PLPLDSFPVP VSTPEGLGTS SNNLGVPATE QRQESFEGFI ARMCSPSPDA
661TSGTGSQRKE EELSRNSRSS SEKKTKTGEY TKKHSDKQHP GKDLHS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

706 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.