Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CEP78 / IP63 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108992-100
Catalog Number: LS-G108992
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEP78 (NM_032171), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C9orf81 | CEP78 | Centrosomal protein of 78 kDa | Centrosomal protein 78kDa | IP63
- Origin Species
- Human
- NCBI GenBank
- AK022705
- SwissProt
- Q5JTW2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIDSVKLRRD SAADFFSHYE YLCALQNSVP LPAVRACLRE GVLDFNADRL RGVDWAPLLS61TLKINKDLPL VSIKSFFQPW LGDTGSDMNK FCRSRVPAIR YKDVTFQLCK ALKGCLSISS121VLKNLELNGL ILRERDLTIL AKGLNKSASL VHLSLANCPI GDGGLEIICQ GIKSSITLKT181VNFTGCNLTW QGADHMAKIL KYQTMRRHEE TWAESLRYRR PDLDCMAGLR RITLNCNTLI241GDLGACAFAD SLSEDLWLRA LDLQQCGLTN EGAKALLEAL ETNTTLVVLD IRKNPLIDHS301MMKAVIKKVL QNGRSAKSEY QWITSPSVKE PSKTAKQKRR TIILGSGHKG KATIRIVGLA361TKKPVSSGRK HSLGKEYYAP APLPPGVSGF LPWRTAERAK RHRGFPLIKT RDICNQLQQP421GFPVTVTVES PSSSEVEEVD DSSESVHEVP EKTSIEQEAL QEKLEECLKQ LKEERVIRLK481VDKRVSELEH ENAQLRNINF SLSEALHAQS LTNMILDDEG VLGSIENSFQ KFHAFLDLLK541DAGLGQLATM AGIDQSDFQL LGHPQMTSTV SNPPKEEKKA LEDEKPEPKQ NALGQMQNIQ601FQKITGDARI PLPLDSFPVP VSTPEGLGTS SNNLGVPATE QRQESFEGFI ARMCSPSPDA661TSGTGSQRKE EELSRNSRSS SEKKTKTGEY TKKHSDKQHP GKDLHSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
706 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.