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Human CDON / Cdo Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CDON / Cdo Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CDON antibodies →
$220.00each
Catalog Number: LS-G80061-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CDON(CDON) (NM_016952) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDON | HPE11 | ORCAM | CDO | Cdon homolog (mouse) | CDON1
Origin Species
Human
NCBI GenBank
NM_016952
SwissProt
Q4KMG0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
136.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHPDLGPLCT LLYVTLTILC SSVSSDLAPY FTSEPLSAVQ KLGGPVVLHC SAQPVTTRIS
61WLHNGKTLDG NLEHVKIHQG TLTILSLNSS LLGYYQCLAN NSIGAIVSGP ATVSVAVLGD
121FGSSTKHVIT AEEKSAGFIG CRVPESNPKA EVRYKIRGKW LEHSTENYLI LPSGNLQILN
181VSLEDKGSYK CAAYNPVTHQ LKVEPIGRKL LVSRPSSDDV HILHPTHSQA LAVLSRSPVT
241LECVVSGVPA PQVYWLKDGQ DIAPGSNWRR LYSHLATDSV DPADSGNYSC MAGNKSGDVK
301YVTYMVNVLE HASISKGLQD QIVSLGATVH FTCDVHGNPA PNCTWFHNAQ PIHPSARHLT
361AGNGLKISGV TVEDVGMYQC VADNGIGFMH STGRLEIEND GGFKPVIITA PVSAKVADGD
421FVTLSCNASG LPVPVIRWYD SHGLITSHPS QVLRSKSRKS QLSRPEGLNL EPVYFVLSQA
481GASSLHIQAV TQEHAGKYIC EAANEHGTTQ AEASLMVVPF ETNTKAETVT LPDAAQNDDR
541SKRDGSETGL LSSFPVKVHP SAVESAPEKN ASGISVPDAP IILSPPQTHT PDTYNLVWRA
601GKDGGLPINA YFVKYRKLDD GVGMLGSWHT VRVPGSENEL HLAELEPSSL YEVLMVARSA
661AGEGQPAMLT FRTSKEKTAS SKNTQASSPP VGIPKYPVVS EAANNNFGVV LTDSSRHSGV
721PEAPDRPTIS TASETSVYVT WIPRANGGSP ITAFKVEYKR MRTSNWLVAA EDIPPSKLSV
781EVRSLEPGST YKFRVIAINH YGESFRSSAS RPYQVVGFPN RFSSRPITGP HIAYTEAVSD
841TQIMLKWTYI PSSNNNTPIQ GFYIYYRPTD SDNDSDYKRD VVEGSKQWHM IGHLQPETSY
901DIKMQCFNEG GESEFSNVMI CETKVKRVPG ASEYPVKDLS TPPNSLGSGG NVGPATSPAR
961SSDMLYLIVG CVLGVMVLIL MVFIAMCLWK NRQQNTIQKY DPPGYLYQGS DMNGQMVDYT
1021TLSGASQING NVHGGFLTNG GLSSGYSHLH HKVPNAVNGI VNGSLNGGLY SGHSNSLTRT
1081HVDFEHPHHL VNGGGMYTAV PQIDPLECVN CRNCRNNNRC FTKTNSTFSS SPPPVVPVVA
1141PYPQDGLEMK PLSHVKVPVC LTSAVPDCGQ LPEESVKDNV EPVPTQRTCC QDIVNDVSSD
1201GSEDPAEFSR GDSCAHSETE INIVSWNALI LPPVPEGCAE KTMWSPPGIP LDSPTEVLQQ
1261PRET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1264 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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