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Human CDH22 / Cadherin 22 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CDH22 / Cadherin 22 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CDH22 antibodies →
$220.00each
Catalog Number: LS-G80476-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Cadherin 22(CDH22) (NM_021248) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cadherin-like 22 | C20orf25 | Cadherin 22 | type 2 | Cadherin-22 | Ortholog of rat PB-cadherin | PB-cadherin | Pituitary and brain cadherin | CDH22 | DJ998H6.1
Origin Species
Human
NCBI GenBank
AF035300
SwissProt
Q9UJ99

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRPRPEGRGL RAGVALSPAL LLLLLLPPPP TLLGRLWAAG TPSPSAPGAR QDGALGAGRV
61KRGWVWNQFF VVEEYTGTEP LYVGKIHSDS DEGDGAIKYT ISGEGAGTIF LIDELTGDIH
121AMERLDREQK TFYTLRAQAR DRATNRLLEP ESEFIIKVQD INDSEPRFLH GPYIGSVAEL
181SPTGTSVMQV MASDADDPTY GSSARLVYSV LDGEHHFTVD PKTGVIRTAV PDLDRESQER
241YEVVIQATDM AGQLGGLSGS TTVTIVVTDV NDNPPRFPQK MYQFSIQESA PIGTAVGRVK
301AEDSDVGENT DMTYHLKDES SSGGDVFKVT TDSDTQEAII VVQKRLDFES QPVHTVILEA
361LNKFVDPRFA DLGTFRDQAI VRVAVTDVDE PPEFRPPSGL LEVQEDAQVG SLVGVVTARD
421PDAANRPVRY AIDRESDLDQ IFDIDADTGA IVTGKGLDRE TAGWHNITVL AMEADNHAQL
481SRASLRIRIL DVNDNPPELA TPYEAAVCED AKPGQLIQTI SVVDRDEPQG GHRFYFRLVP
541EAPSNPHFSL LDIQDNTAAV HTQHVGFNRQ EQDVFFLPIL VVDSGPPTLS STGTLTIRIC
601GCDSSGTIQS CNTTAFVMAA SLSPGALIAL LVCVLILVVL VLLILTLRRH HKSHLSSDED
661EDMRDNVIKY NDEGGGEQDT EAYDMSALRS LYDFGELKGG DGGGSAGGGA GGGSGGGAGS
721PPQAHLPSER HSLPQGPPSP EPDFSVFRDF ISRKVALADG DLSVPPYDAF QTYAFEGADS
781PAASLSSLHS GSSGSEQDFA YLSSWGPRFR PLAALYAGHR GDDEAQAS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

828 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.