Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH22 / Cadherin 22 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110690-100
Catalog Number: LS-G110690
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cadherin 22(CDH22) (NM_021248), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin-like 22 | C20orf25 | Cadherin 22 | type 2 | Cadherin-22 | Ortholog of rat PB-cadherin | PB-cadherin | Pituitary and brain cadherin | CDH22 | DJ998H6.1
- Origin Species
- Human
- NCBI GenBank
- AF035300
- SwissProt
- Q9UJ99
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRPRPEGRGL RAGVALSPAL LLLLLLPPPP TLLGRLWAAG TPSPSAPGAR QDGALGAGRV61KRGWVWNQFF VVEEYTGTEP LYVGKIHSDS DEGDGAIKYT ISGEGAGTIF LIDELTGDIH121AMERLDREQK TFYTLRAQAR DRATNRLLEP ESEFIIKVQD INDSEPRFLH GPYIGSVAEL181SPTGTSVMQV MASDADDPTY GSSARLVYSV LDGEHHFTVD PKTGVIRTAV PDLDRESQER241YEVVIQATDM AGQLGGLSGS TTVTIVVTDV NDNPPRFPQK MYQFSIQESA PIGTAVGRVK301AEDSDVGENT DMTYHLKDES SSGGDVFKVT TDSDTQEAII VVQKRLDFES QPVHTVILEA361LNKFVDPRFA DLGTFRDQAI VRVAVTDVDE PPEFRPPSGL LEVQEDAQVG SLVGVVTARD421PDAANRPVRY AIDRESDLDQ IFDIDADTGA IVTGKGLDRE TAGWHNITVL AMEADNHAQL481SRASLRIRIL DVNDNPPELA TPYEAAVCED AKPGQLIQTI SVVDRDEPQG GHRFYFRLVP541EAPSNPHFSL LDIQDNTAAV HTQHVGFNRQ EQDVFFLPIL VVDSGPPTLS STGTLTIRIC601GCDSSGTIQS CNTTAFVMAA SLSPGALIAL LVCVLILVVL VLLILTLRRH HKSHLSSDED661EDMRDNVIKY NDEGGGEQDT EAYDMSALRS LYDFGELKGG DGGGSAGGGA GGGSGGGAGS721PPQAHLPSER HSLPQGPPSP EPDFSVFRDF ISRKVALADG DLSVPPYDAF QTYAFEGADS781PAASLSSLHS GSSGSEQDFA YLSSWGPRFR PLAALYAGHR GDDEAQASTRTRPLEQKLISEEDLAANDILDYKDDDDKV
828 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.