CDC42BPB / MRCKB Antibody
Catalog Number: LS-C485312-100
100 µL / $309.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CDC42BPB / MRCKB Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G82556-20
Catalog Number: LS-G82556
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CDC42 Binding Protein Kinase Beta(CDC42BPB) (NM_006035) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDC42BP-beta | CDC42-binding kinase beta | CDC42BPB | DMPK-like beta | MRCK beta | KIAA1124 | MRCKB
- Origin Species
- Hu
- NCBI GenBank
- NM_006035
- SwissProt
- Q9Y5S2
Protein
- Expression System
- HEK293T
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 191.65 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSAKVRLKKL EQLLLDGPWR NESALSVETL LDVLVCLYTE CSHSALRRDK YVAEFLEWAK61PFTQLVKEMQ LHREDFEIIK VIGRGAFGEV AVVKMKNTER IYAMKILNKW EMLKRAETAC121FREERDVLVN GDCQWITALH YAFQDENHLY LVMDYYVGGD LLTLLSKFED KLPEDMARFY181IGEMVLAIDS IHQLHYVHRD IKPDNVLLDV NGHIRLADFG SCLKMNDDGT VQSSVAVGTP241DYISPEILQA MEDGMGKYGP ECDWWSLGVC MYEMLYGETP FYAESLVETY GKIMNHEERF301QFPSHVTDVS EEAKDLIQRL ICSRERRLGQ NGIEDFKKHA FFEGLNWENI RNLEAPYIPD361VSSPSDTSNF DVDDDVLRNT EILPPGSHTG FSGLHLPFIG FTFTTESCFS DRGSLKSIMQ421SNTLTKDEDV QRDLEHSLQM EAYERRIRRL EQEKLELSRK LQESTQTVQS LHGSSRALSN481SNRDKEIKKL NEEIERLKNK IADSNRLERQ LEDTVALRQE REDSTQRLRG LEKQHRVVRQ541EKEELHKQLV EASERLKSQA KELKDAHQQR KLALQEFSEL NERMAELRAQ KQKVSRQLRD601KEEEMEVATQ KVDAMRQEMR RAEKLRKELE AQLDDAVAEA SKERKLREHS ENFCKQMESE661LEALKVKQGG RGAGATLEHQ QEISKIKSEL EKKVLFYEEE LVRREASHVL EVKNVKKEVH721DSESHQLALQ KEILMLKDKL EKSKRERHNE MEEAVGTIKD KYERERAMLF DENKKLTAEN781EKLCSFVDKL TAQNRQLEDE LQDLAAKKES VAHWEAQIAE IIQWVSDEKD ARGYLQALAS841KMTEELEALR SSSLGSRTLD PLWKVRRSQK LDMSARLELQ SALEAEIRAK QLVQEELRKV901KDANLTLESK LKDSEAKNRE LLEEMEILKK KMEEKFRADT GLKLPDFQDS IFEYFNTAPL961AHDLTFRTSS ASEQETQAPK PEASPSMSVA ASEQQEPKAH QFSIKSFSSP TQCSHCTSLM1021VGLIRQGYAC EVCSFACHVS CKDGAPQVCP IPPEQSKRPL GVDVQRGIGT AYKGHVKVPK1081PTGVKKGWQR AYAVVCDCKL FLYDLPEGKS TQPGVIASQV LDLRDDEFSV SSVLASDVIH1141ATRRDIPCIF RVTASLLGAP SKTSSLLILT ENENEKRKWV GILEGLQSIL HKNRLRNQVV1201HVPLEAYDSS LPLIKAILTA AIVDADRIAV GLEEGLYVIE VTRDVIVRAA DCKKVHQIEL1261APREKIVILL CGRNHHVHLY PWSSLDGAEG SFDIKLPETK GCQLMATATL KRNSGTCLFV1321AVKRLILCYE IQRTKPFHRK FNEIVAPGSV QCLAVLRDRL CVGYPSGFCL LSIQGDGQPL1381NLVNPNDPSL AFLSQQSFDA LCAVELESEE YLLCFSHMGL YVDPQGRRAR AQELMWPAAP1441VACSCSPTHV TVYSEYGVDV FDVRTMEWVQ TIGLRRIRPL NSEGTLNLLN CEPPRLIYFK1501SKFSGAVLNV PDTSDNSKKQ MLRTRSKRRF VFKVPEEERL QQRREMLRDP ELRSKMISNP1561TNFNHVAHMG PGDGMQVLMD LPLSAVPPSQ EERPGPAPTN LARQPPSRNK PYISWPSSGG1621SEPSVTVPLR SMSDPDQDFD KEPDSDSTKH STPSNSSNPS GPPSPNSPHR SQLPLEGLEQ1681PACDTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1685 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
