LSBio
Human CDC42BPB / MRCKB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CDC42BPB / MRCKB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CDC42BPB antibodies →
$220.00each
Catalog Number: LS-G82556-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CDC42 Binding Protein Kinase Beta(CDC42BPB) (NM_006035) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDC42BP-beta | CDC42-binding kinase beta | CDC42BPB | DMPK-like beta | MRCK beta | KIAA1124 | MRCKB
Origin Species
Human
NCBI GenBank
NM_006035
SwissProt
Q9Y5S2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
191.65 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAKVRLKKL EQLLLDGPWR NESALSVETL LDVLVCLYTE CSHSALRRDK YVAEFLEWAK
61PFTQLVKEMQ LHREDFEIIK VIGRGAFGEV AVVKMKNTER IYAMKILNKW EMLKRAETAC
121FREERDVLVN GDCQWITALH YAFQDENHLY LVMDYYVGGD LLTLLSKFED KLPEDMARFY
181IGEMVLAIDS IHQLHYVHRD IKPDNVLLDV NGHIRLADFG SCLKMNDDGT VQSSVAVGTP
241DYISPEILQA MEDGMGKYGP ECDWWSLGVC MYEMLYGETP FYAESLVETY GKIMNHEERF
301QFPSHVTDVS EEAKDLIQRL ICSRERRLGQ NGIEDFKKHA FFEGLNWENI RNLEAPYIPD
361VSSPSDTSNF DVDDDVLRNT EILPPGSHTG FSGLHLPFIG FTFTTESCFS DRGSLKSIMQ
421SNTLTKDEDV QRDLEHSLQM EAYERRIRRL EQEKLELSRK LQESTQTVQS LHGSSRALSN
481SNRDKEIKKL NEEIERLKNK IADSNRLERQ LEDTVALRQE REDSTQRLRG LEKQHRVVRQ
541EKEELHKQLV EASERLKSQA KELKDAHQQR KLALQEFSEL NERMAELRAQ KQKVSRQLRD
601KEEEMEVATQ KVDAMRQEMR RAEKLRKELE AQLDDAVAEA SKERKLREHS ENFCKQMESE
661LEALKVKQGG RGAGATLEHQ QEISKIKSEL EKKVLFYEEE LVRREASHVL EVKNVKKEVH
721DSESHQLALQ KEILMLKDKL EKSKRERHNE MEEAVGTIKD KYERERAMLF DENKKLTAEN
781EKLCSFVDKL TAQNRQLEDE LQDLAAKKES VAHWEAQIAE IIQWVSDEKD ARGYLQALAS
841KMTEELEALR SSSLGSRTLD PLWKVRRSQK LDMSARLELQ SALEAEIRAK QLVQEELRKV
901KDANLTLESK LKDSEAKNRE LLEEMEILKK KMEEKFRADT GLKLPDFQDS IFEYFNTAPL
961AHDLTFRTSS ASEQETQAPK PEASPSMSVA ASEQQEPKAH QFSIKSFSSP TQCSHCTSLM
1021VGLIRQGYAC EVCSFACHVS CKDGAPQVCP IPPEQSKRPL GVDVQRGIGT AYKGHVKVPK
1081PTGVKKGWQR AYAVVCDCKL FLYDLPEGKS TQPGVIASQV LDLRDDEFSV SSVLASDVIH
1141ATRRDIPCIF RVTASLLGAP SKTSSLLILT ENENEKRKWV GILEGLQSIL HKNRLRNQVV
1201HVPLEAYDSS LPLIKAILTA AIVDADRIAV GLEEGLYVIE VTRDVIVRAA DCKKVHQIEL
1261APREKIVILL CGRNHHVHLY PWSSLDGAEG SFDIKLPETK GCQLMATATL KRNSGTCLFV
1321AVKRLILCYE IQRTKPFHRK FNEIVAPGSV QCLAVLRDRL CVGYPSGFCL LSIQGDGQPL
1381NLVNPNDPSL AFLSQQSFDA LCAVELESEE YLLCFSHMGL YVDPQGRRAR AQELMWPAAP
1441VACSCSPTHV TVYSEYGVDV FDVRTMEWVQ TIGLRRIRPL NSEGTLNLLN CEPPRLIYFK
1501SKFSGAVLNV PDTSDNSKKQ MLRTRSKRRF VFKVPEEERL QQRREMLRDP ELRSKMISNP
1561TNFNHVAHMG PGDGMQVLMD LPLSAVPPSQ EERPGPAPTN LARQPPSRNK PYISWPSSGG
1621SEPSVTVPLR SMSDPDQDFD KEPDSDSTKH STPSNSSNPS GPPSPNSPHR SQLPLEGLEQ
1681PACDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1685 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.