Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDC42BPB / MRCKB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115571-100
Catalog Number: LS-G115571
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDC42 Binding Protein Kinase Beta(CDC42BPB) (NM_006035), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDC42BP-beta | CDC42-binding kinase beta | CDC42BPB | DMPK-like beta | MRCK beta | KIAA1124 | MRCKB
- Origin Species
- Human
- NCBI GenBank
- NM_006035
- SwissProt
- Q9Y5S2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 191.65 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSAKVRLKKL EQLLLDGPWR NESALSVETL LDVLVCLYTE CSHSALRRDK YVAEFLEWAK61PFTQLVKEMQ LHREDFEIIK VIGRGAFGEV AVVKMKNTER IYAMKILNKW EMLKRAETAC121FREERDVLVN GDCQWITALH YAFQDENHLY LVMDYYVGGD LLTLLSKFED KLPEDMARFY181IGEMVLAIDS IHQLHYVHRD IKPDNVLLDV NGHIRLADFG SCLKMNDDGT VQSSVAVGTP241DYISPEILQA MEDGMGKYGP ECDWWSLGVC MYEMLYGETP FYAESLVETY GKIMNHEERF301QFPSHVTDVS EEAKDLIQRL ICSRERRLGQ NGIEDFKKHA FFEGLNWENI RNLEAPYIPD361VSSPSDTSNF DVDDDVLRNT EILPPGSHTG FSGLHLPFIG FTFTTESCFS DRGSLKSIMQ421SNTLTKDEDV QRDLEHSLQM EAYERRIRRL EQEKLELSRK LQESTQTVQS LHGSSRALSN481SNRDKEIKKL NEEIERLKNK IADSNRLERQ LEDTVALRQE REDSTQRLRG LEKQHRVVRQ541EKEELHKQLV EASERLKSQA KELKDAHQQR KLALQEFSEL NERMAELRAQ KQKVSRQLRD601KEEEMEVATQ KVDAMRQEMR RAEKLRKELE AQLDDAVAEA SKERKLREHS ENFCKQMESE661LEALKVKQGG RGAGATLEHQ QEISKIKSEL EKKVLFYEEE LVRREASHVL EVKNVKKEVH721DSESHQLALQ KEILMLKDKL EKSKRERHNE MEEAVGTIKD KYERERAMLF DENKKLTAEN781EKLCSFVDKL TAQNRQLEDE LQDLAAKKES VAHWEAQIAE IIQWVSDEKD ARGYLQALAS841KMTEELEALR SSSLGSRTLD PLWKVRRSQK LDMSARLELQ SALEAEIRAK QLVQEELRKV901KDANLTLESK LKDSEAKNRE LLEEMEILKK KMEEKFRADT GLKLPDFQDS IFEYFNTAPL961AHDLTFRTSS ASEQETQAPK PEASPSMSVA ASEQQEPKAH QFSIKSFSSP TQCSHCTSLM1021VGLIRQGYAC EVCSFACHVS CKDGAPQVCP IPPEQSKRPL GVDVQRGIGT AYKGHVKVPK1081PTGVKKGWQR AYAVVCDCKL FLYDLPEGKS TQPGVIASQV LDLRDDEFSV SSVLASDVIH1141ATRRDIPCIF RVTASLLGAP SKTSSLLILT ENENEKRKWV GILEGLQSIL HKNRLRNQVV1201HVPLEAYDSS LPLIKAILTA AIVDADRIAV GLEEGLYVIE VTRDVIVRAA DCKKVHQIEL1261APREKIVILL CGRNHHVHLY PWSSLDGAEG SFDIKLPETK GCQLMATATL KRNSGTCLFV1321AVKRLILCYE IQRTKPFHRK FNEIVAPGSV QCLAVLRDRL CVGYPSGFCL LSIQGDGQPL1381NLVNPNDPSL AFLSQQSFDA LCAVELESEE YLLCFSHMGL YVDPQGRRAR AQELMWPAAP1441VACSCSPTHV TVYSEYGVDV FDVRTMEWVQ TIGLRRIRPL NSEGTLNLLN CEPPRLIYFK1501SKFSGAVLNV PDTSDNSKKQ MLRTRSKRRF VFKVPEEERL QQRREMLRDP ELRSKMISNP1561TNFNHVAHMG PGDGMQVLMD LPLSAVPPSQ EERPGPAPTN LARQPPSRNK PYISWPSSGG1621SEPSVTVPLR SMSDPDQDFD KEPDSDSTKH STPSNSSNPS GPPSPNSPHR SQLPLEGLEQ1681PACDTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1685 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.