CCDC77 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G108889-100
100 µg (1 mg/mL) / $504.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CCDC77 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G83755-20
Catalog Number: LS-G83755
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CCDC77 (NM_032358) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCDC77
- Origin Species
- Human
- NCBI GenBank
- NM_032358
- SwissProt
- Q9BR77
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNFTPTHTPV CRKRTVVSKR GVAVSGPTKR RGMADSLEST PLPSPEDRLA KLHPSKELLE61YYQKKMAECE AENEDLLKKL ELYKEACEGQ HKLECDLQQR EEEIAELQKA LSDMQVCLFQ121EREHVLRLYS ENDRLRIREL EDKKKIQNLL ALVGTDAGEV TYFCKEPPHK VTILQKTIQA181VGECEQSESS AFKADPKISK RRPSRERKES SEHYQRDIQT LILQVEALQA QLGEQTKLSR241EQIEGLIEDR RIHLEEIQVQ HQRNQNKIKE LTKNLHHTQE LLYESTKDFL QLRSENQNKE301KSWMLEKDNL MSKIKQYRVQ CKKKEDKIGK VLPVMHESHH AQSEYIKSLK DKLVQEKKLS361NMYQEQCISL EEELARIREE EGMRREIFKD RTNKMGKRLQ IMTKRYEALE RRRILEVEGF421KTDIKVLRQK LKDLEQMLYK ATVNARANQD LALLCEVRDS NRRAHKIQGE LKNLKSKVFG481LENELRLCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
488 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
