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CCDC77 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC77 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC77 antibodies →
$504.00each
Catalog Number: LS-G108889-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC77 (NM_032358), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC77
Origin Species
Human
NCBI GenBank
NM_032358
SwissProt
Q9BR77

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNFTPTHTPV CRKRTVVSKR GVAVSGPTKR RGMADSLEST PLPSPEDRLA KLHPSKELLE
61YYQKKMAECE AENEDLLKKL ELYKEACEGQ HKLECDLQQR EEEIAELQKA LSDMQVCLFQ
121EREHVLRLYS ENDRLRIREL EDKKKIQNLL ALVGTDAGEV TYFCKEPPHK VTILQKTIQA
181VGECEQSESS AFKADPKISK RRPSRERKES SEHYQRDIQT LILQVEALQA QLGEQTKLSR
241EQIEGLIEDR RIHLEEIQVQ HQRNQNKIKE LTKNLHHTQE LLYESTKDFL QLRSENQNKE
301KSWMLEKDNL MSKIKQYRVQ CKKKEDKIGK VLPVMHESHH AQSEYIKSLK DKLVQEKKLS
361NMYQEQCISL EEELARIREE EGMRREIFKD RTNKMGKRLQ IMTKRYEALE RRRILEVEGF
421KTDIKVLRQK LKDLEQMLYK ATVNARANQD LALLCEVRDS NRRAHKIQGE LKNLKSKVFG
481LENELRLC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

488 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.