Human CCDC185 Protein (Recombinant His, N-Terminal)
Catalog Number: LS-G144276-50
50 µg / $428.00
Expression System Escherichia coli (Prokaryotic)
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CCDC185 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93629-20
Catalog Number: LS-G93629
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C1orf65(CCDC185) (NM_152610) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C1orf65 | RP11-76K24.1
- Origin Species
- Human
- SwissProt
- Q8N715
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGFSHFSQP PYRDLWEPPR PGGERESTQR LGGQRSGADS TACSRAGTPG AESEAGACWL61HPHCSFTPRP RRRGCSDSLR GSRSLSDVAR RPLERSRKHR PRSRRLEDAW GETGTKPRPA121WQPQTQLPPQ RPQPCPHYPL AQGDSPPPCP GGAGTPLSGT FRVEKAQGGD QWAVPLGRHL181GRWSPSSVPS ERSSVPSQKF KRHSACVCAQ KRDSSDQVES LASRDSQPLA SSKEMRSPHT241QVLKSKLEEV VVSSQDQQIV ALVLTRLKKA QRIRELQQQA AKAWEELKRS DQKVQMTLER301ERRLLLRQSQ EQWQEKEQRK TLQSPEQRDL RRDSQRKNVP PGESRWKEQP EDQESPRQEK361LEKARAQAEH RKQCQVRRLL EQEKMLRNLR EQHSLQLQRR LVEACRKRHL HAVEGQKKVQ421DTNLSSLINY QARKVLMDCQ AKAEELLRQL SLEQSFQRSQ EIHQGLRKER QRELREKAQK481EEEQLQQARW RAGESEEQRK MRKRILVELA DEKIRQARSH VHKTTRDKVQ HLRELNHLRE541KNHHILKLKA EKEEKCHIEG IKEAIKKKEQ RVQHISQGKD PNFQEFQKLP QASRREERAP601PNSSLDQMVL EAQLRACQQN RGYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
623 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
