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CCDC185 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC185 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC185 antibodies →
$504.00each
Catalog Number: LS-G106141-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf65(CCDC185) (NM_152610), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C1orf65 | RP11-76K24.1
Origin Species
Human
SwissProt
Q8N715

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGFSHFSQP PYRDLWEPPR PGGERESTQR LGGQRSGADS TACSRAGTPG AESEAGACWL
61HPHCSFTPRP RRRGCSDSLR GSRSLSDVAR RPLERSRKHR PRSRRLEDAW GETGTKPRPA
121WQPQTQLPPQ RPQPCPHYPL AQGDSPPPCP GGAGTPLSGT FRVEKAQGGD QWAVPLGRHL
181GRWSPSSVPS ERSSVPSQKF KRHSACVCAQ KRDSSDQVES LASRDSQPLA SSKEMRSPHT
241QVLKSKLEEV VVSSQDQQIV ALVLTRLKKA QRIRELQQQA AKAWEELKRS DQKVQMTLER
301ERRLLLRQSQ EQWQEKEQRK TLQSPEQRDL RRDSQRKNVP PGESRWKEQP EDQESPRQEK
361LEKARAQAEH RKQCQVRRLL EQEKMLRNLR EQHSLQLQRR LVEACRKRHL HAVEGQKKVQ
421DTNLSSLINY QARKVLMDCQ AKAEELLRQL SLEQSFQRSQ EIHQGLRKER QRELREKAQK
481EEEQLQQARW RAGESEEQRK MRKRILVELA DEKIRQARSH VHKTTRDKVQ HLRELNHLRE
541KNHHILKLKA EKEEKCHIEG IKEAIKKKEQ RVQHISQGKD PNFQEFQKLP QASRREERAP
601PNSSLDQMVL EAQLRACQQN RGY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

623 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.