LSBio
Human CBFA1 / RUNX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CBFA1 / RUNX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all RUNX2 antibodies →
$220.00each
Catalog Number: LS-G81231-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RUNX2 (NM_001024630) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AML3 | CCD | CCD1 | OSF2 | PEBP2A1 | PEBP2A2 | PEA2-alpha A | PEBP2-alpha A | OSF-2 | PEBP2A | PEBP2aA1 | RUNX2 | CBF-alpha-1 | CBFA1 | Oncogene AML-3 | PEA2aA | PEBP2aA
Origin Species
Human
NCBI GenBank
NM_004348
SwissProt
Q13950

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.44 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLHSPHKQPQ NHKCGANFLQ EDSKKSLVFK WLISAGHYQP PRPTESFKAA SSIYNRGYKF
61YLKKKGGTMA SNSLFSTVTP CQQNFFWDPS TSRRFSPPSS SLQPGKMSDV SPVVAAQQQQ
121QQQQQQQQQQ QQQQQQQQQE AAAAAAAAAA AAAAAAAVPR LRPPHDNRTM VEIIADHPAE
181LVRTDSPNFL CSVLPSHWRC NKTLPVAFKV VALGEVPDGT VVTVMAGNDE NYSAELRNAS
241AVMKNQVARF NDLRFVGRSG RGKSFTLTIT VFTNPPQVAT YHRAIKVTVD GPREPRRHRQ
301KLDDSKPSLF SDRLSDLGRI PHPSMRVGVP PQNPRPSLNS APSPFNPQGQ SQITDPRQAQ
361SSPPWSYDQS YPSYLSQMTS PSIHSTTPLS STRGTGLPAI TDVPRRISDD DTATSDFCLW
421PSTLSKKSQA GASELGPFSD PRQFPSISSL TESRFSNPRM HYPATFTYTP PVTSGMSLGM
481SATTHYHTYL PPPYPGSSQS QSGPFQTSST PYLYYGTSSG SYQFPMVPGG DRSPSRMLPP
541CTTTSNGSTL LNPNLPNQND GVDADGSHSS SPTVLNSSGR MDESVWRPY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

589 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.