Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CBFA1 / RUNX2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99287-100
Catalog Number: LS-G99287
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RUNX2 (NM_001024630), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AML3 | CCD | CCD1 | OSF2 | PEBP2A1 | PEBP2A2 | PEA2-alpha A | PEBP2-alpha A | OSF-2 | PEBP2A | PEBP2aA1 | RUNX2 | CBF-alpha-1 | CBFA1 | Oncogene AML-3 | PEA2aA | PEBP2aA
- NCBI GenBank
- NM_004348
- SwissProt
- Q13950
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.44 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLHSPHKQPQ NHKCGANFLQ EDSKKSLVFK WLISAGHYQP PRPTESFKAA SSIYNRGYKF61YLKKKGGTMA SNSLFSTVTP CQQNFFWDPS TSRRFSPPSS SLQPGKMSDV SPVVAAQQQQ121QQQQQQQQQQ QQQQQQQQQE AAAAAAAAAA AAAAAAAVPR LRPPHDNRTM VEIIADHPAE181LVRTDSPNFL CSVLPSHWRC NKTLPVAFKV VALGEVPDGT VVTVMAGNDE NYSAELRNAS241AVMKNQVARF NDLRFVGRSG RGKSFTLTIT VFTNPPQVAT YHRAIKVTVD GPREPRRHRQ301KLDDSKPSLF SDRLSDLGRI PHPSMRVGVP PQNPRPSLNS APSPFNPQGQ SQITDPRQAQ361SSPPWSYDQS YPSYLSQMTS PSIHSTTPLS STRGTGLPAI TDVPRRISDD DTATSDFCLW421PSTLSKKSQA GASELGPFSD PRQFPSISSL TESRFSNPRM HYPATFTYTP PVTSGMSLGM481SATTHYHTYL PPPYPGSSQS QSGPFQTSST PYLYYGTSSG SYQFPMVPGG DRSPSRMLPP541CTTTSNGSTL LNPNLPNQND GVDADGSHSS SPTVLNSSGR MDESVWRPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
589 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.