CAPN15 / SOLH Antibody
Catalog Number: LS-C831185
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CAPN15 / SOLH Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G85602-20
Catalog Number: LS-G85602
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 15(CAPN15) (NM_005632) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAPN15 | Calpain-15 | SOLH | Small optic lobes homolog
- Origin Species
- Human
- NCBI GenBank
- NM_005632
- SwissProt
- O75808
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 117.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATVGEWSCV RCTFLNPAGQ RQCSICEAPR HKPDLNHILR LSVEEQKWPC ARCTFRNFLG61KEACEVCGFT PEPAPGAAFL PVLNGVLPKP PAILGEPKGS CQEEAGPVRT AGLVATEPAR121GQCEDKDEEE KEEQEEEEGA AEPRGGWACP RCTLHNTPVA SSCSVCGGPR RLSLPRIPPE181ALVVPEVVAP AGFHVVPAAP PPGLPGEGAE ANPPATSQGP AAEPEPPRVP PFSPFSSTLQ241NNPVPRSRRE VPPQLQPPVP EAAQPSPSAG CRGAPQGSGW AGASRLAELL SGKRLSVLEE301EATEGGTSRV EAGSSTSGSD IIDLAGDTVR YTPASPSSPD FTTWSCAKCT LRNPTVAPRC361SACGCSKLHG FQEHGEPPTH CPDCGADKPS PCGRSCGRVS SAQKAARVLP ERPGQWACPA421CTLLNALRAK HCAACHTPQL LVAQRRGAAP LRRRESMHVE QRRQTDEGEA KALWENIVAF481CRENNVSFVD DSFPPGPESV GFPAGDSVQQ RVRQWLRPQE INCSVFRDHR ATWSVFHTLR541PSDILQGLLG NCWFLSALAV LAERPDLVER VMVTRSLCAE GAYQVRLCKD GTWTTVLVDD601MLPCDEAGCL LFSQAQRKQL WVALIEKALA KLHGSYFALQ AGRAIEGLAT LTGAPCESLA661LQLSSTNPRE EPVDTDLIWA KMLSSKEAGF LMGASCGGGN MKVDDSAYES LGLRPRHAYS721ILDVRDVQGT RLLRLRNPWG RFSWNGSWSD EWPHWPGHLR GELMPHGSSE GVFWMEYGDF781VRYFDSVDIC KVHSDWQEAR VQGCFPSSAS APVGVTALTV LERASLEFAL FQEGSRRSDA841VDSHLLDLCI LVFRATFGSG GHLSLGRLLA HSKRAVKKFV SCDVMLEPGE YAVVCCAFNH901WGPPLPGTPA PQASSPSAGV PRASPEPPGH VLAVYSSRLV MVEPVEAQPT TLADAIILLT961ESRGERHEGR EGMTCYYLTH GWAGLIVVVE NRHPKAYLHV QCDCTDSFNV VSTRGSLRTQ1021DSVPPLHRQV LVILSQLEGN AGFSITHRLA HRKAAQAFLS DWTASKGTHS PPLTPEVAGL1081HGPRPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1086 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
