Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAPN15 / SOLH Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115827-100
Catalog Number: LS-G115827
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 15(CAPN15) (NM_005632), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAPN15 | Calpain-15 | SOLH | Small optic lobes homolog
- Origin Species
- Human
- NCBI GenBank
- NM_005632
- SwissProt
- O75808
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 117.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATVGEWSCV RCTFLNPAGQ RQCSICEAPR HKPDLNHILR LSVEEQKWPC ARCTFRNFLG61KEACEVCGFT PEPAPGAAFL PVLNGVLPKP PAILGEPKGS CQEEAGPVRT AGLVATEPAR121GQCEDKDEEE KEEQEEEEGA AEPRGGWACP RCTLHNTPVA SSCSVCGGPR RLSLPRIPPE181ALVVPEVVAP AGFHVVPAAP PPGLPGEGAE ANPPATSQGP AAEPEPPRVP PFSPFSSTLQ241NNPVPRSRRE VPPQLQPPVP EAAQPSPSAG CRGAPQGSGW AGASRLAELL SGKRLSVLEE301EATEGGTSRV EAGSSTSGSD IIDLAGDTVR YTPASPSSPD FTTWSCAKCT LRNPTVAPRC361SACGCSKLHG FQEHGEPPTH CPDCGADKPS PCGRSCGRVS SAQKAARVLP ERPGQWACPA421CTLLNALRAK HCAACHTPQL LVAQRRGAAP LRRRESMHVE QRRQTDEGEA KALWENIVAF481CRENNVSFVD DSFPPGPESV GFPAGDSVQQ RVRQWLRPQE INCSVFRDHR ATWSVFHTLR541PSDILQGLLG NCWFLSALAV LAERPDLVER VMVTRSLCAE GAYQVRLCKD GTWTTVLVDD601MLPCDEAGCL LFSQAQRKQL WVALIEKALA KLHGSYFALQ AGRAIEGLAT LTGAPCESLA661LQLSSTNPRE EPVDTDLIWA KMLSSKEAGF LMGASCGGGN MKVDDSAYES LGLRPRHAYS721ILDVRDVQGT RLLRLRNPWG RFSWNGSWSD EWPHWPGHLR GELMPHGSSE GVFWMEYGDF781VRYFDSVDIC KVHSDWQEAR VQGCFPSSAS APVGVTALTV LERASLEFAL FQEGSRRSDA841VDSHLLDLCI LVFRATFGSG GHLSLGRLLA HSKRAVKKFV SCDVMLEPGE YAVVCCAFNH901WGPPLPGTPA PQASSPSAGV PRASPEPPGH VLAVYSSRLV MVEPVEAQPT TLADAIILLT961ESRGERHEGR EGMTCYYLTH GWAGLIVVVE NRHPKAYLHV QCDCTDSFNV VSTRGSLRTQ1021DSVPPLHRQV LVILSQLEGN AGFSITHRLA HRKAAQAFLS DWTASKGTHS PPLTPEVAGL1081HGPRPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1086 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.