CAPN12 / Calpain 12 Antibody
Catalog Number: LS-C659041-100
100 µL / $394.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human CAPN12 / Calpain 12 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G94301-20
Catalog Number: LS-G94301
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 12(CAPN12) (NM_144691) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAPN12 | Calpain-12 | CANP 12 | Calpain 12
- Origin Species
- Human
- SwissProt
- Q6ZSI9
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSSGRVTI QLVDEEAGVG AGRLQLFRGQ SYEAIRAACL DSGILFRDPY FPAGPDALGY61DQLGPDSEKA KGVKWMRPHE FCAEPKFICE DMSRTDVCQG SLGNCWFLAA AASLTLYPRL121LRRVVPPGQD FQHGYAGVFH FQLWQFGRWM DVVVDDRLPV REGKLMFVRS EQRNEFWAPL181LEKAYAKLHG SYEVMRGGHM NEAFVDFTGG VGEVLYLRQN SMGLFSALRH ALAKESLVGA241TALSDRGEYR TEEGLVKGHA YSITGTHKVF LGFTKVRLLR LRNPWGCVEW TGAWSDSCPR301WDTLPTECRD ALLVKKEDGE FWMELRDFLL HFDTVQICSL SPEVLGPSPE GGGWHVHTFQ361GRWVRGFNSG GSQPNAETFW TNPQFRLTLL EPDEEDDEDE EGPWGGWGAA GARGPARGGR421TPKCTVLLSL IQRNRRRLRA KGLTYLTVGF HVFQIPEELL GLWDSPRSHA LLPRLLRADR481SPLSARRDVT RRCCLRPGHY LVVPSTAHAG DEADFTLRVF SERRHTAVEI DDVISADLQS541LQGPYLPLEL GLEQLFQELA GEEEELNASQ LQALLSIALE PARAHTSTPR EIGLRTCEQL601LQCFGHGQSL ALHHFQQLWG YLLEWQAIFN KFDEDTSGTM NSYELRLALN AAGFHLNNQL661TQTLTSRYRD SRLRVDFERF VSCVAHLTCI FCHCSQHLDG GEGVICLTHR QWMEVATFSSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
719 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
