Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAPN12 / Calpain 12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106898-100
Catalog Number: LS-G106898
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 12(CAPN12) (NM_144691), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAPN12 | Calpain-12 | CANP 12 | Calpain 12
- Origin Species
- Human
- SwissProt
- Q6ZSI9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSSGRVTI QLVDEEAGVG AGRLQLFRGQ SYEAIRAACL DSGILFRDPY FPAGPDALGY61DQLGPDSEKA KGVKWMRPHE FCAEPKFICE DMSRTDVCQG SLGNCWFLAA AASLTLYPRL121LRRVVPPGQD FQHGYAGVFH FQLWQFGRWM DVVVDDRLPV REGKLMFVRS EQRNEFWAPL181LEKAYAKLHG SYEVMRGGHM NEAFVDFTGG VGEVLYLRQN SMGLFSALRH ALAKESLVGA241TALSDRGEYR TEEGLVKGHA YSITGTHKVF LGFTKVRLLR LRNPWGCVEW TGAWSDSCPR301WDTLPTECRD ALLVKKEDGE FWMELRDFLL HFDTVQICSL SPEVLGPSPE GGGWHVHTFQ361GRWVRGFNSG GSQPNAETFW TNPQFRLTLL EPDEEDDEDE EGPWGGWGAA GARGPARGGR421TPKCTVLLSL IQRNRRRLRA KGLTYLTVGF HVFQIPEELL GLWDSPRSHA LLPRLLRADR481SPLSARRDVT RRCCLRPGHY LVVPSTAHAG DEADFTLRVF SERRHTAVEI DDVISADLQS541LQGPYLPLEL GLEQLFQELA GEEEELNASQ LQALLSIALE PARAHTSTPR EIGLRTCEQL601LQCFGHGQSL ALHHFQQLWG YLLEWQAIFN KFDEDTSGTM NSYELRLALN AAGFHLNNQL661TQTLTSRYRD SRLRVDFERF VSCVAHLTCI FCHCSQHLDG GEGVICLTHR QWMEVATFSSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
719 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.